Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 30491 | 5' | -59.2 | NC_006548.1 | + | 16714 | 0.67 | 0.304373 |
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Target: 5'- cGCagGCCGCCGUCCUggucauagCGGaAGCg- -3' miRNA: 3'- aCGg-CGGCGGUAGGAaa------GCCgUCGac -5' |
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| 30491 | 5' | -59.2 | NC_006548.1 | + | 33704 | 0.68 | 0.282088 |
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Target: 5'- cGCUGUCGCUGUCCUgcCGGCAa--- -3' miRNA: 3'- aCGGCGGCGGUAGGAaaGCCGUcgac -5' |
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| 30491 | 5' | -59.2 | NC_006548.1 | + | 29744 | 0.68 | 0.282088 |
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Target: 5'- aGCCaGCCGCCA-CUac-CGGCAGCc- -3' miRNA: 3'- aCGG-CGGCGGUaGGaaaGCCGUCGac -5' |
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| 30491 | 5' | -59.2 | NC_006548.1 | + | 22614 | 0.68 | 0.261116 |
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Target: 5'- gGCCGUCGaUCAggcucUCCUccgcCGGCGGCUGu -3' miRNA: 3'- aCGGCGGC-GGU-----AGGAaa--GCCGUCGAC- -5' |
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| 30491 | 5' | -59.2 | NC_006548.1 | + | 27291 | 0.69 | 0.247852 |
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Target: 5'- gGCUGCCGCC------UCGGCAGCg- -3' miRNA: 3'- aCGGCGGCGGuaggaaAGCCGUCGac -5' |
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| 30491 | 5' | -59.2 | NC_006548.1 | + | 19396 | 0.71 | 0.179636 |
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Target: 5'- aGCuCGCUGCCGUCCagcUGGUAGCg- -3' miRNA: 3'- aCG-GCGGCGGUAGGaaaGCCGUCGac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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