Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30491 | 5' | -59.2 | NC_006548.1 | + | 3459 | 0.66 | 0.370285 |
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Target: 5'- cGCCGUgGCCuucaccagGUUCUUUCGaGCguccaGGCUGg -3' miRNA: 3'- aCGGCGgCGG--------UAGGAAAGC-CG-----UCGAC- -5' |
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| 30491 | 5' | -59.2 | NC_006548.1 | + | 20337 | 0.66 | 0.37918 |
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Target: 5'- gGgCGCCGUCAUCC--UCGGCgaAGUUc -3' miRNA: 3'- aCgGCGGCGGUAGGaaAGCCG--UCGAc -5' |
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| 30491 | 5' | -59.2 | NC_006548.1 | + | 24990 | 0.66 | 0.37918 |
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Target: 5'- cGCCGCCgacGCCAaCCUa--GGUGGCUu -3' miRNA: 3'- aCGGCGG---CGGUaGGAaagCCGUCGAc -5' |
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| 30491 | 5' | -59.2 | NC_006548.1 | + | 2439 | 0.66 | 0.385491 |
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Target: 5'- aGCCGgcucggauggaaaaCCGCCggCCUggCGGguGCg- -3' miRNA: 3'- aCGGC--------------GGCGGuaGGAaaGCCguCGac -5' |
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| 30491 | 5' | -59.2 | NC_006548.1 | + | 8197 | 0.66 | 0.388217 |
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Target: 5'- aGCgG-CGCUAUCCagUUcCGGCAGCUc -3' miRNA: 3'- aCGgCgGCGGUAGGa-AA-GCCGUCGAc -5' |
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| 30491 | 5' | -59.2 | NC_006548.1 | + | 21014 | 0.66 | 0.391871 |
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Target: 5'- cGCCGUCGCCGgauuucucggaaaagUCCaccugUUUgGGCAGUUc -3' miRNA: 3'- aCGGCGGCGGU---------------AGG-----AAAgCCGUCGAc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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