Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 30491 | 5' | -59.2 | NC_006548.1 | + | 16714 | 0.67 | 0.304373 |
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Target: 5'- cGCagGCCGCCGUCCUggucauagCGGaAGCg- -3' miRNA: 3'- aCGg-CGGCGGUAGGAaa------GCCgUCGac -5' |
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| 30491 | 5' | -59.2 | NC_006548.1 | + | 8197 | 0.66 | 0.388217 |
|
Target: 5'- aGCgG-CGCUAUCCagUUcCGGCAGCUc -3' miRNA: 3'- aCGgCgGCGGUAGGa-AA-GCCGUCGAc -5' |
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| 30491 | 5' | -59.2 | NC_006548.1 | + | 4653 | 0.67 | 0.33946 |
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Target: 5'- uUGCUGCCGUgGaaaagcaccuggaguUCCgcUUCGGCcgGGCUGg -3' miRNA: 3'- -ACGGCGGCGgU---------------AGGa-AAGCCG--UCGAC- -5' |
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| 30491 | 5' | -59.2 | NC_006548.1 | + | 3459 | 0.66 | 0.370285 |
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Target: 5'- cGCCGUgGCCuucaccagGUUCUUUCGaGCguccaGGCUGg -3' miRNA: 3'- aCGGCGgCGG--------UAGGAAAGC-CG-----UCGAC- -5' |
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| 30491 | 5' | -59.2 | NC_006548.1 | + | 2439 | 0.66 | 0.385491 |
|
Target: 5'- aGCCGgcucggauggaaaaCCGCCggCCUggCGGguGCg- -3' miRNA: 3'- aCGGC--------------GGCGGuaGGAaaGCCguCGac -5' |
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| 30491 | 5' | -59.2 | NC_006548.1 | + | 552 | 1.07 | 0.000285 |
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Target: 5'- cUGCCGCCGCCAUCCUUUCGGCAGCUGu -3' miRNA: 3'- -ACGGCGGCGGUAGGAAAGCCGUCGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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