Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 30498 | 5' | -57.1 | NC_006548.1 | + | 21893 | 0.68 | 0.43312 |
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Target: 5'- gGCCucGCGAUCGAGCAggaucagguUgGugGUCUGc -3' miRNA: 3'- -CGGc-CGCUAGCUCGU---------GgCugCAGACu -5' |
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| 30498 | 5' | -57.1 | NC_006548.1 | + | 33626 | 0.68 | 0.43312 |
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Target: 5'- cGCUGuCGAUCGccaAGCGCuCGACGUCg-- -3' miRNA: 3'- -CGGCcGCUAGC---UCGUG-GCUGCAGacu -5' |
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| 30498 | 5' | -57.1 | NC_006548.1 | + | 20078 | 0.69 | 0.369685 |
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Target: 5'- gGCCGGCGcAUUGAGCuuggggugGCCGAUGacgagcggggccucgCUGAg -3' miRNA: 3'- -CGGCCGC-UAGCUCG--------UGGCUGCa--------------GACU- -5' |
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| 30498 | 5' | -57.1 | NC_006548.1 | + | 6021 | 0.69 | 0.351681 |
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Target: 5'- aCCGGUGAUCGGGCA--GACGUucagccgucaggCUGAg -3' miRNA: 3'- cGGCCGCUAGCUCGUggCUGCA------------GACU- -5' |
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| 30498 | 5' | -57.1 | NC_006548.1 | + | 22781 | 0.71 | 0.288908 |
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Target: 5'- gGCCGGCa--CGAGCACCuucuGCGcCUGAa -3' miRNA: 3'- -CGGCCGcuaGCUCGUGGc---UGCaGACU- -5' |
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| 30498 | 5' | -57.1 | NC_006548.1 | + | 12636 | 0.72 | 0.247851 |
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Target: 5'- aGuuGGCGGcCGAGCGcCCGGCGU-UGAa -3' miRNA: 3'- -CggCCGCUaGCUCGU-GGCUGCAgACU- -5' |
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| 30498 | 5' | -57.1 | NC_006548.1 | + | 11747 | 0.66 | 0.567486 |
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Target: 5'- uGCgCGGCGcgCGgcGGCGCCGGgGUUg-- -3' miRNA: 3'- -CG-GCCGCuaGC--UCGUGGCUgCAGacu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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