Results 1 - 20 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30520 | 5' | -59.6 | NC_006548.1 | + | 25436 | 0.66 | 0.388195 |
Target: 5'- aCAUCAgauccuUGCCagGCCGuGCGcGCAGGCc- -3' miRNA: 3'- -GUAGU------ACGGg-CGGC-CGUaCGUCCGcc -5' |
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30520 | 5' | -59.6 | NC_006548.1 | + | 35946 | 0.66 | 0.379258 |
Target: 5'- -----aGUUgGCCGGCAgGguGGCGGc -3' miRNA: 3'- guaguaCGGgCGGCCGUaCguCCGCC- -5' |
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30520 | 5' | -59.6 | NC_006548.1 | + | 4281 | 0.66 | 0.379258 |
Target: 5'- gCAUCggGCCagcuCCGGCGucagcacggUGCAGaGCGGc -3' miRNA: 3'- -GUAGuaCGGgc--GGCCGU---------ACGUC-CGCC- -5' |
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30520 | 5' | -59.6 | NC_006548.1 | + | 21983 | 0.66 | 0.361802 |
Target: 5'- gCGUCAUccugcGCCCGaacauCGGCGUGCuGGGCc- -3' miRNA: 3'- -GUAGUA-----CGGGCg----GCCGUACG-UCCGcc -5' |
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30520 | 5' | -59.6 | NC_006548.1 | + | 21739 | 0.67 | 0.344912 |
Target: 5'- --cCAcgGCCuggacgCGCCGGUgccGCAGGCGGa -3' miRNA: 3'- guaGUa-CGG------GCGGCCGua-CGUCCGCC- -5' |
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30520 | 5' | -59.6 | NC_006548.1 | + | 36704 | 0.67 | 0.336682 |
Target: 5'- gAUCA-GCgCGCCGGCcucAUGCGGcGCuGGc -3' miRNA: 3'- gUAGUaCGgGCGGCCG---UACGUC-CG-CC- -5' |
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30520 | 5' | -59.6 | NC_006548.1 | + | 25862 | 0.67 | 0.328596 |
Target: 5'- uGUCAccGCCUGCUGGCccgcGCGGGCc- -3' miRNA: 3'- gUAGUa-CGGGCGGCCGua--CGUCCGcc -5' |
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30520 | 5' | -59.6 | NC_006548.1 | + | 15489 | 0.67 | 0.328596 |
Target: 5'- uCAUCAUGCCCuggaucucaucGUCGGCcgGCAG-Ca- -3' miRNA: 3'- -GUAGUACGGG-----------CGGCCGuaCGUCcGcc -5' |
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30520 | 5' | -59.6 | NC_006548.1 | + | 17762 | 0.67 | 0.312858 |
Target: 5'- -----aGUCCGCC-GUAUGCGGcGCGGg -3' miRNA: 3'- guaguaCGGGCGGcCGUACGUC-CGCC- -5' |
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30520 | 5' | -59.6 | NC_006548.1 | + | 2327 | 0.67 | 0.305205 |
Target: 5'- -uUCcUGUUCGUCGGC-UGCGcGGCGGc -3' miRNA: 3'- guAGuACGGGCGGCCGuACGU-CCGCC- -5' |
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30520 | 5' | -59.6 | NC_006548.1 | + | 22733 | 0.68 | 0.290334 |
Target: 5'- -cUCGUGCCgGCCGGCgAUGaaguGGCc- -3' miRNA: 3'- guAGUACGGgCGGCCG-UACgu--CCGcc -5' |
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30520 | 5' | -59.6 | NC_006548.1 | + | 20866 | 0.68 | 0.283113 |
Target: 5'- gAUCAccggaGCCUucugGCgCGGCA-GCAGGCGGc -3' miRNA: 3'- gUAGUa----CGGG----CG-GCCGUaCGUCCGCC- -5' |
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30520 | 5' | -59.6 | NC_006548.1 | + | 4137 | 0.7 | 0.218601 |
Target: 5'- uCGUCA-GCCUGCuCGGCGucgaUGUGGGCuGGa -3' miRNA: 3'- -GUAGUaCGGGCG-GCCGU----ACGUCCG-CC- -5' |
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30520 | 5' | -59.6 | NC_006548.1 | + | 23866 | 0.7 | 0.218601 |
Target: 5'- gGUCGcGCCa-CCGGCAUgGCcGGCGGg -3' miRNA: 3'- gUAGUaCGGgcGGCCGUA-CGuCCGCC- -5' |
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30520 | 5' | -59.6 | NC_006548.1 | + | 24518 | 0.7 | 0.201856 |
Target: 5'- gGUCuUGCCgCGCugcaagCGGCcauauUGCAGGCGGu -3' miRNA: 3'- gUAGuACGG-GCG------GCCGu----ACGUCCGCC- -5' |
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30520 | 5' | -59.6 | NC_006548.1 | + | 18517 | 0.71 | 0.186236 |
Target: 5'- cCGUCGUGgCCGCCGGgucCAUGUcGGCa- -3' miRNA: 3'- -GUAGUACgGGCGGCC---GUACGuCCGcc -5' |
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30520 | 5' | -59.6 | NC_006548.1 | + | 17032 | 0.71 | 0.181271 |
Target: 5'- -----aGCuCCGCCuGCAUGCcGGCGGg -3' miRNA: 3'- guaguaCG-GGCGGcCGUACGuCCGCC- -5' |
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30520 | 5' | -59.6 | NC_006548.1 | + | 27020 | 0.71 | 0.162567 |
Target: 5'- gAUCAgcagacgGCCCGCaucagGGcCAUGguGGCGGc -3' miRNA: 3'- gUAGUa------CGGGCGg----CC-GUACguCCGCC- -5' |
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30520 | 5' | -59.6 | NC_006548.1 | + | 29889 | 0.72 | 0.153881 |
Target: 5'- gGUCAUGCCCgGCUGGguUGC-GGCc- -3' miRNA: 3'- gUAGUACGGG-CGGCCguACGuCCGcc -5' |
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30520 | 5' | -59.6 | NC_006548.1 | + | 19954 | 0.72 | 0.149698 |
Target: 5'- --aCGUGCCgGCCGGCGgggaggauugGCAgaguGGCGGu -3' miRNA: 3'- guaGUACGGgCGGCCGUa---------CGU----CCGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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