Results 1 - 20 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30521 | 3' | -51.4 | NC_006548.1 | + | 14036 | 0.66 | 0.828462 |
Target: 5'- uCCGCCgcgagcugGACUACuuGGCCgacCGUGACc -3' miRNA: 3'- -GGCGG--------CUGAUGuuCUGGaacGUACUG- -5' |
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30521 | 3' | -51.4 | NC_006548.1 | + | 5017 | 0.66 | 0.818812 |
Target: 5'- -aG-CGAUaccaaggGCAAGACCUUGCGUGuCa -3' miRNA: 3'- ggCgGCUGa------UGUUCUGGAACGUACuG- -5' |
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30521 | 3' | -51.4 | NC_006548.1 | + | 32853 | 0.66 | 0.818812 |
Target: 5'- cCCGCUGGCgggauuuucgUGCuggGGGCCUUGCGcuGCu -3' miRNA: 3'- -GGCGGCUG----------AUGu--UCUGGAACGUacUG- -5' |
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30521 | 3' | -51.4 | NC_006548.1 | + | 15420 | 0.66 | 0.818812 |
Target: 5'- gCCgGCCGACgAUGAGAUCcaggGCAUGGa -3' miRNA: 3'- -GG-CGGCUGaUGUUCUGGaa--CGUACUg -5' |
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30521 | 3' | -51.4 | NC_006548.1 | + | 35875 | 0.66 | 0.798869 |
Target: 5'- aUGCCGGCgcgauccGCGcGGCCggcgGCGUGAUa -3' miRNA: 3'- gGCGGCUGa------UGUuCUGGaa--CGUACUG- -5' |
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30521 | 3' | -51.4 | NC_006548.1 | + | 36903 | 0.66 | 0.798869 |
Target: 5'- aCCGCaacCGGCUGCugcAGGCCgucaacUGguUGACu -3' miRNA: 3'- -GGCG---GCUGAUGu--UCUGGa-----ACguACUG- -5' |
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30521 | 3' | -51.4 | NC_006548.1 | + | 8816 | 0.66 | 0.795809 |
Target: 5'- gUCGCaCGACgucgcccaaggaugUGCAgcaguugcAGACCguccUGCAUGACg -3' miRNA: 3'- -GGCG-GCUG--------------AUGU--------UCUGGa---ACGUACUG- -5' |
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30521 | 3' | -51.4 | NC_006548.1 | + | 16594 | 0.67 | 0.788602 |
Target: 5'- aCGCCGA--ACAGcAUCUUGCugGUGACg -3' miRNA: 3'- gGCGGCUgaUGUUcUGGAACG--UACUG- -5' |
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30521 | 3' | -51.4 | NC_006548.1 | + | 22787 | 0.67 | 0.778153 |
Target: 5'- aUCGCCGGCcggcACGAGcACCUucUGCGccugaaUGGCa -3' miRNA: 3'- -GGCGGCUGa---UGUUC-UGGA--ACGU------ACUG- -5' |
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30521 | 3' | -51.4 | NC_006548.1 | + | 7813 | 0.67 | 0.778153 |
Target: 5'- gCUGCCGcgaGCUgcGCAAGGCUcUGCGcGACc -3' miRNA: 3'- -GGCGGC---UGA--UGUUCUGGaACGUaCUG- -5' |
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30521 | 3' | -51.4 | NC_006548.1 | + | 3722 | 0.67 | 0.767536 |
Target: 5'- -aGCUGAggucGCAuAGGCUUUGCGUGGCg -3' miRNA: 3'- ggCGGCUga--UGU-UCUGGAACGUACUG- -5' |
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30521 | 3' | -51.4 | NC_006548.1 | + | 23502 | 0.67 | 0.767536 |
Target: 5'- aUCGCCGAggGCAAGuucaGCCUcggGCcgGAUg -3' miRNA: 3'- -GGCGGCUgaUGUUC----UGGAa--CGuaCUG- -5' |
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30521 | 3' | -51.4 | NC_006548.1 | + | 16972 | 0.67 | 0.767536 |
Target: 5'- cCCGCCGGCaUGCAggcGGAgCUgcuggaaGCcgGGCg -3' miRNA: 3'- -GGCGGCUG-AUGU---UCUgGAa------CGuaCUG- -5' |
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30521 | 3' | -51.4 | NC_006548.1 | + | 23803 | 0.67 | 0.767536 |
Target: 5'- cCCGCCGGCcaugccggugGCGcGACCcgGCAgaUGGCa -3' miRNA: 3'- -GGCGGCUGa---------UGUuCUGGaaCGU--ACUG- -5' |
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30521 | 3' | -51.4 | NC_006548.1 | + | 24514 | 0.67 | 0.767536 |
Target: 5'- uUGCCGcGCUGCAAGcgGCCauaUUGCA-GGCg -3' miRNA: 3'- gGCGGC-UGAUGUUC--UGG---AACGUaCUG- -5' |
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30521 | 3' | -51.4 | NC_006548.1 | + | 29172 | 0.67 | 0.766465 |
Target: 5'- aCCGCCGAC--CAGGuagagcaacuuguGCCUcGCAUGGa -3' miRNA: 3'- -GGCGGCUGauGUUC-------------UGGAaCGUACUg -5' |
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30521 | 3' | -51.4 | NC_006548.1 | + | 12278 | 0.68 | 0.723654 |
Target: 5'- cCCGUCGGCgcACAGGGCCUggcGCAg--- -3' miRNA: 3'- -GGCGGCUGa-UGUUCUGGAa--CGUacug -5' |
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30521 | 3' | -51.4 | NC_006548.1 | + | 14107 | 0.68 | 0.712397 |
Target: 5'- uUCGCCGAUUu---GACCcgGUAUGGCa -3' miRNA: 3'- -GGCGGCUGAuguuCUGGaaCGUACUG- -5' |
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30521 | 3' | -51.4 | NC_006548.1 | + | 3896 | 0.69 | 0.665468 |
Target: 5'- aUCGCCGgccacuucuccaaGCUGCAugcGGAgCUgcagUGCAUGGCg -3' miRNA: 3'- -GGCGGC-------------UGAUGU---UCUgGA----ACGUACUG- -5' |
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30521 | 3' | -51.4 | NC_006548.1 | + | 14937 | 0.7 | 0.620269 |
Target: 5'- -gGCCGAuCUGcCGGGACUUcUGCAUGAg -3' miRNA: 3'- ggCGGCU-GAU-GUUCUGGA-ACGUACUg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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