Results 1 - 20 of 29 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30557 | 3' | -54.3 | NC_006548.1 | + | 31432 | 0.66 | 0.637804 |
Target: 5'- aCGGCGGCcuauCUGC---ACCUGCAcgUCg -3' miRNA: 3'- cGCCGUCGuu--GACGaucUGGACGU--AG- -5' |
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30557 | 3' | -54.3 | NC_006548.1 | + | 20690 | 0.66 | 0.637804 |
Target: 5'- aGCGcG-AGCAACUGCUGGccACCcGCGa- -3' miRNA: 3'- -CGC-CgUCGUUGACGAUC--UGGaCGUag -5' |
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30557 | 3' | -54.3 | NC_006548.1 | + | 28563 | 0.66 | 0.637804 |
Target: 5'- cGCuGGUccuGCcAUUGCUGGAUCUGCGc- -3' miRNA: 3'- -CG-CCGu--CGuUGACGAUCUGGACGUag -5' |
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30557 | 3' | -54.3 | NC_006548.1 | + | 9577 | 0.66 | 0.600796 |
Target: 5'- cGCGcaccagaGCAGCcuccuGCUGgUGGACCgaaccguUGCAUCa -3' miRNA: 3'- -CGC-------CGUCGu----UGACgAUCUGG-------ACGUAG- -5' |
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30557 | 3' | -54.3 | NC_006548.1 | + | 28077 | 0.66 | 0.591578 |
Target: 5'- gGCGGCuGGCGGcCUGCaGGccgaacucaaccGCCUGCAg- -3' miRNA: 3'- -CGCCG-UCGUU-GACGaUC------------UGGACGUag -5' |
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30557 | 3' | -54.3 | NC_006548.1 | + | 29355 | 0.66 | 0.590427 |
Target: 5'- cGCGGUcucgccgauGGCAcccugucGCUGCaggAGGCCgcgGUAUCg -3' miRNA: 3'- -CGCCG---------UCGU-------UGACGa--UCUGGa--CGUAG- -5' |
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30557 | 3' | -54.3 | NC_006548.1 | + | 20595 | 0.67 | 0.56865 |
Target: 5'- aGCGGCAGaGGCUGaCcGGACCUcucacaCAUCa -3' miRNA: 3'- -CGCCGUCgUUGAC-GaUCUGGAc-----GUAG- -5' |
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30557 | 3' | -54.3 | NC_006548.1 | + | 22753 | 0.67 | 0.566368 |
Target: 5'- aUGGCAGCGacgaGCUGCUcGAUCgccagcguggcgGCGUCc -3' miRNA: 3'- cGCCGUCGU----UGACGAuCUGGa-----------CGUAG- -5' |
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30557 | 3' | -54.3 | NC_006548.1 | + | 17534 | 0.67 | 0.557266 |
Target: 5'- aGCaGGCaAGCAGCuUGgaAGAUCUGCGc- -3' miRNA: 3'- -CG-CCG-UCGUUG-ACgaUCUGGACGUag -5' |
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30557 | 3' | -54.3 | NC_006548.1 | + | 7768 | 0.67 | 0.544821 |
Target: 5'- cGCGGCAGCucaugcGCUcgaugucgucuagGUcgAGGCCUGCAa- -3' miRNA: 3'- -CGCCGUCGu-----UGA-------------CGa-UCUGGACGUag -5' |
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30557 | 3' | -54.3 | NC_006548.1 | + | 16343 | 0.68 | 0.512478 |
Target: 5'- cGUGGCGGCAAC-----GACCUGCGg- -3' miRNA: 3'- -CGCCGUCGUUGacgauCUGGACGUag -5' |
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30557 | 3' | -54.3 | NC_006548.1 | + | 22916 | 0.68 | 0.501506 |
Target: 5'- cGCGGCGGCGA--GUUGGGCCggacUAUCg -3' miRNA: 3'- -CGCCGUCGUUgaCGAUCUGGac--GUAG- -5' |
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30557 | 3' | -54.3 | NC_006548.1 | + | 2794 | 0.68 | 0.501506 |
Target: 5'- cCGGCAGCGugcuugccCUGCgccGCCUGCAgaUCg -3' miRNA: 3'- cGCCGUCGUu-------GACGaucUGGACGU--AG- -5' |
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30557 | 3' | -54.3 | NC_006548.1 | + | 13576 | 0.68 | 0.4874 |
Target: 5'- gGCGGCGGCaAGCUGCUgcucaaccagauccAGaaaaGCCUGgAUUc -3' miRNA: 3'- -CGCCGUCG-UUGACGA--------------UC----UGGACgUAG- -5' |
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30557 | 3' | -54.3 | NC_006548.1 | + | 32466 | 0.68 | 0.470301 |
Target: 5'- aCGGCGGCGACgGCUGccGGCCaggcugaugccaucgGCGUCc -3' miRNA: 3'- cGCCGUCGUUGaCGAU--CUGGa--------------CGUAG- -5' |
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30557 | 3' | -54.3 | NC_006548.1 | + | 35265 | 0.68 | 0.469242 |
Target: 5'- cUGGCGGCGAUggugggcgGcCUGGuACCUGCGUUc -3' miRNA: 3'- cGCCGUCGUUGa-------C-GAUC-UGGACGUAG- -5' |
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30557 | 3' | -54.3 | NC_006548.1 | + | 33315 | 0.68 | 0.469242 |
Target: 5'- uGCGGCGcugcGCAGcCUGCUGuacGcCCUGCGUg -3' miRNA: 3'- -CGCCGU----CGUU-GACGAU---CuGGACGUAg -5' |
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30557 | 3' | -54.3 | NC_006548.1 | + | 37841 | 0.69 | 0.438074 |
Target: 5'- gGUGGCuGCuccgAACgugGCUGGugCUGCAaaUCa -3' miRNA: 3'- -CGCCGuCG----UUGa--CGAUCugGACGU--AG- -5' |
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30557 | 3' | -54.3 | NC_006548.1 | + | 23112 | 0.71 | 0.352232 |
Target: 5'- aCGGCAGCAACUGagcaGCCUGgGUg -3' miRNA: 3'- cGCCGUCGUUGACgaucUGGACgUAg -5' |
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30557 | 3' | -54.3 | NC_006548.1 | + | 13210 | 0.71 | 0.352232 |
Target: 5'- uCGGuCAGUuGCUGCUGuACCUGCAg- -3' miRNA: 3'- cGCC-GUCGuUGACGAUcUGGACGUag -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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