Results 1 - 20 of 28 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30571 | 5' | -59.5 | NC_006548.1 | + | 18723 | 0.66 | 0.394964 |
Target: 5'- uGGGCAgCCcugcggcgguuGUCGCCAUggcuuucgCAUCGCGCa- -3' miRNA: 3'- cCCCGU-GG-----------UAGCGGUA--------GUGGCGCGac -5' |
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30571 | 5' | -59.5 | NC_006548.1 | + | 18634 | 0.66 | 0.368331 |
Target: 5'- cGGGCuuCCA-CGCCA--GCUGCGCUc -3' miRNA: 3'- cCCCGu-GGUaGCGGUagUGGCGCGAc -5' |
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30571 | 5' | -59.5 | NC_006548.1 | + | 36677 | 0.66 | 0.385949 |
Target: 5'- -uGGCACCGuaUCGaCgCAUCcCCGCGCg- -3' miRNA: 3'- ccCCGUGGU--AGC-G-GUAGuGGCGCGac -5' |
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30571 | 5' | -59.5 | NC_006548.1 | + | 20520 | 0.66 | 0.394964 |
Target: 5'- uGGGCACUggCaCCGugggugacugcUCGCCGCGCg- -3' miRNA: 3'- cCCCGUGGuaGcGGU-----------AGUGGCGCGac -5' |
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30571 | 5' | -59.5 | NC_006548.1 | + | 28542 | 0.67 | 0.359731 |
Target: 5'- -cGGCGCCG--GCCAggUCGCgGCGCUa -3' miRNA: 3'- ccCCGUGGUagCGGU--AGUGgCGCGAc -5' |
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30571 | 5' | -59.5 | NC_006548.1 | + | 32725 | 0.67 | 0.318866 |
Target: 5'- cGGGGUgacaggcgcaGCgAUCGCCGUUGCucgacucccgaCGUGCUGg -3' miRNA: 3'- -CCCCG----------UGgUAGCGGUAGUG-----------GCGCGAC- -5' |
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30571 | 5' | -59.5 | NC_006548.1 | + | 35509 | 0.67 | 0.326753 |
Target: 5'- cGGGguUCAUCGCCuucaccugGUCGCCGgccagGCUGa -3' miRNA: 3'- cCCCguGGUAGCGG--------UAGUGGCg----CGAC- -5' |
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30571 | 5' | -59.5 | NC_006548.1 | + | 13005 | 0.67 | 0.326753 |
Target: 5'- -cGGCugCGcCGCaCAgCACCGCGCUc -3' miRNA: 3'- ccCCGugGUaGCG-GUaGUGGCGCGAc -5' |
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30571 | 5' | -59.5 | NC_006548.1 | + | 16282 | 0.67 | 0.358879 |
Target: 5'- cGGGCuuuuccaucaauaGCCuccaaaGUCGUUAUUACCGCGCa- -3' miRNA: 3'- cCCCG-------------UGG------UAGCGGUAGUGGCGCGac -5' |
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30571 | 5' | -59.5 | NC_006548.1 | + | 28281 | 0.67 | 0.363154 |
Target: 5'- aGGGGCugCGggCGUCGUCAacgucaccaaguucgUCGCGgaGg -3' miRNA: 3'- -CCCCGugGUa-GCGGUAGU---------------GGCGCgaC- -5' |
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30571 | 5' | -59.5 | NC_006548.1 | + | 23375 | 0.67 | 0.350434 |
Target: 5'- cGGGGUugucaccggguggACCAggGCCAUCGCU-CGCUa -3' miRNA: 3'- -CCCCG-------------UGGUagCGGUAGUGGcGCGAc -5' |
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30571 | 5' | -59.5 | NC_006548.1 | + | 8125 | 0.67 | 0.326753 |
Target: 5'- aGGGagGCCGUCGCCga-ACUGCGCc- -3' miRNA: 3'- cCCCg-UGGUAGCGGuagUGGCGCGac -5' |
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30571 | 5' | -59.5 | NC_006548.1 | + | 12794 | 0.67 | 0.315752 |
Target: 5'- -cGGCACCugagcuguauugcUCGCCAgUGCCGCGCg- -3' miRNA: 3'- ccCCGUGGu------------AGCGGUaGUGGCGCGac -5' |
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30571 | 5' | -59.5 | NC_006548.1 | + | 37333 | 0.68 | 0.303524 |
Target: 5'- aGGGGCG-CGUCGCCA-CGCUGC-Cg- -3' miRNA: 3'- -CCCCGUgGUAGCGGUaGUGGCGcGac -5' |
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30571 | 5' | -59.5 | NC_006548.1 | + | 11689 | 0.68 | 0.303524 |
Target: 5'- -cGGCGCCGcCGCg--CGCCGCGCa- -3' miRNA: 3'- ccCCGUGGUaGCGguaGUGGCGCGac -5' |
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30571 | 5' | -59.5 | NC_006548.1 | + | 4737 | 0.68 | 0.296069 |
Target: 5'- uGGGCACCAa-GCUuUCcCCGCGCa- -3' miRNA: 3'- cCCCGUGGUagCGGuAGuGGCGCGac -5' |
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30571 | 5' | -59.5 | NC_006548.1 | + | 20558 | 0.69 | 0.25432 |
Target: 5'- aGGGCACCGUCaccCCAUCcgACgGUGCUu -3' miRNA: 3'- cCCCGUGGUAGc--GGUAG--UGgCGCGAc -5' |
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30571 | 5' | -59.5 | NC_006548.1 | + | 20401 | 0.69 | 0.235321 |
Target: 5'- aGGcGGCGCaggAUCucggucagaGCCGUCACCGCGUa- -3' miRNA: 3'- -CC-CCGUGg--UAG---------CGGUAGUGGCGCGac -5' |
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30571 | 5' | -59.5 | NC_006548.1 | + | 31712 | 0.69 | 0.24785 |
Target: 5'- aGGGGcCGCCAUCcagGCCAUC-CagGCGCa- -3' miRNA: 3'- -CCCC-GUGGUAG---CGGUAGuGg-CGCGac -5' |
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30571 | 5' | -59.5 | NC_006548.1 | + | 27522 | 0.69 | 0.24785 |
Target: 5'- aGGGGCugCuguUCGCCGgcaUCGCCG-GUUa -3' miRNA: 3'- -CCCCGugGu--AGCGGU---AGUGGCgCGAc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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