Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 30571 | 5' | -59.5 | NC_006548.1 | + | 35509 | 0.67 | 0.326753 |
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Target: 5'- cGGGguUCAUCGCCuucaccugGUCGCCGgccagGCUGa -3' miRNA: 3'- cCCCguGGUAGCGG--------UAGUGGCg----CGAC- -5' |
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| 30571 | 5' | -59.5 | NC_006548.1 | + | 32725 | 0.67 | 0.318866 |
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Target: 5'- cGGGGUgacaggcgcaGCgAUCGCCGUUGCucgacucccgaCGUGCUGg -3' miRNA: 3'- -CCCCG----------UGgUAGCGGUAGUG-----------GCGCGAC- -5' |
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| 30571 | 5' | -59.5 | NC_006548.1 | + | 4737 | 0.68 | 0.296069 |
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Target: 5'- uGGGCACCAa-GCUuUCcCCGCGCa- -3' miRNA: 3'- cCCCGUGGUagCGGuAGuGGCGCGac -5' |
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| 30571 | 5' | -59.5 | NC_006548.1 | + | 31712 | 0.69 | 0.24785 |
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Target: 5'- aGGGGcCGCCAUCcagGCCAUC-CagGCGCa- -3' miRNA: 3'- -CCCC-GUGGUAG---CGGUAGuGg-CGCGac -5' |
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| 30571 | 5' | -59.5 | NC_006548.1 | + | 20401 | 0.69 | 0.235321 |
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Target: 5'- aGGcGGCGCaggAUCucggucagaGCCGUCACCGCGUa- -3' miRNA: 3'- -CC-CCGUGg--UAG---------CGGUAGUGGCGCGac -5' |
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| 30571 | 5' | -59.5 | NC_006548.1 | + | 21396 | 0.71 | 0.175639 |
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Target: 5'- uGGGCgucgGCCGUCaGCgCAUCACCGCGagUGa -3' miRNA: 3'- cCCCG----UGGUAG-CG-GUAGUGGCGCg-AC- -5' |
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| 30571 | 5' | -59.5 | NC_006548.1 | + | 17516 | 0.75 | 0.100811 |
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Target: 5'- cGGccaGCugCggCGCCAUCGCCGCuGCUGg -3' miRNA: 3'- -CCc--CGugGuaGCGGUAGUGGCG-CGAC- -5' |
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| 30571 | 5' | -59.5 | NC_006548.1 | + | 29415 | 0.76 | 0.082596 |
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Target: 5'- aGGGUGCCAUCGgCGagACCGCGCa- -3' miRNA: 3'- cCCCGUGGUAGCgGUagUGGCGCGac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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