Results 1 - 20 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30590 | 3' | -60 | NC_006549.1 | + | 129969 | 0.66 | 0.71259 |
Target: 5'- uAUUGGUgC-CCAGGCCCUaCGaacgcAGGGCg -3' miRNA: 3'- uUGGCCAgGuGGUCCGGGA-GU-----UCCUG- -5' |
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30590 | 3' | -60 | NC_006549.1 | + | 7416 | 0.66 | 0.702665 |
Target: 5'- -cCCGGgcCCGCCcGGCCC-CucGGACc -3' miRNA: 3'- uuGGCCa-GGUGGuCCGGGaGuuCCUG- -5' |
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30590 | 3' | -60 | NC_006549.1 | + | 6756 | 0.66 | 0.682648 |
Target: 5'- cGCgGGUCCGCaaGGGCCCgacggagaCAAGGGu -3' miRNA: 3'- uUGgCCAGGUGg-UCCGGGa-------GUUCCUg -5' |
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30590 | 3' | -60 | NC_006549.1 | + | 44045 | 0.66 | 0.672573 |
Target: 5'- cGACCGGgCUACCAcGCCCaccaAAGGAUc -3' miRNA: 3'- -UUGGCCaGGUGGUcCGGGag--UUCCUG- -5' |
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30590 | 3' | -60 | NC_006549.1 | + | 131439 | 0.66 | 0.662467 |
Target: 5'- cGGCCGucuUCCACCAuuccGGCCa-CAGGGGCu -3' miRNA: 3'- -UUGGCc--AGGUGGU----CCGGgaGUUCCUG- -5' |
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30590 | 3' | -60 | NC_006549.1 | + | 102136 | 0.67 | 0.652336 |
Target: 5'- cGAUUGGUCCugggggGCCaggAGGUCCUgGGGGGCc -3' miRNA: 3'- -UUGGCCAGG------UGG---UCCGGGAgUUCCUG- -5' |
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30590 | 3' | -60 | NC_006549.1 | + | 40941 | 0.67 | 0.642189 |
Target: 5'- cACgGGcCCcCCGGGCCCgauGGGGCc -3' miRNA: 3'- uUGgCCaGGuGGUCCGGGaguUCCUG- -5' |
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30590 | 3' | -60 | NC_006549.1 | + | 7121 | 0.67 | 0.642189 |
Target: 5'- -uUUGGuUCCGCCGGGCCCau--GGGCc -3' miRNA: 3'- uuGGCC-AGGUGGUCCGGGaguuCCUG- -5' |
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30590 | 3' | -60 | NC_006549.1 | + | 123903 | 0.67 | 0.632035 |
Target: 5'- ----uGUCCACCucuucGGCCUUCGAGGAa -3' miRNA: 3'- uuggcCAGGUGGu----CCGGGAGUUCCUg -5' |
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30590 | 3' | -60 | NC_006549.1 | + | 8323 | 0.67 | 0.611734 |
Target: 5'- cACUGGUCCccACCAcGCCgCcCAAGGACc -3' miRNA: 3'- uUGGCCAGG--UGGUcCGG-GaGUUCCUG- -5' |
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30590 | 3' | -60 | NC_006549.1 | + | 7530 | 0.68 | 0.581412 |
Target: 5'- -cCCGGU-----GGGCCCUCAGGGGCc -3' miRNA: 3'- uuGGCCAgguggUCCGGGAGUUCCUG- -5' |
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30590 | 3' | -60 | NC_006549.1 | + | 41016 | 0.68 | 0.541513 |
Target: 5'- cGCaagaGGUCCGaUAGGCCCcCAGGGAUc -3' miRNA: 3'- uUGg---CCAGGUgGUCCGGGaGUUCCUG- -5' |
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30590 | 3' | -60 | NC_006549.1 | + | 7005 | 0.69 | 0.525801 |
Target: 5'- uGCCGGcgcUCCGggaccucaaggagcuCCcGGUCCUCAAGGGCc -3' miRNA: 3'- uUGGCC---AGGU---------------GGuCCGGGAGUUCCUG- -5' |
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30590 | 3' | -60 | NC_006549.1 | + | 50494 | 0.7 | 0.446616 |
Target: 5'- cAGCCGGgCCAUCuGGCCCcgCGGGGcCa -3' miRNA: 3'- -UUGGCCaGGUGGuCCGGGa-GUUCCuG- -5' |
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30590 | 3' | -60 | NC_006549.1 | + | 7202 | 0.7 | 0.441211 |
Target: 5'- gGGCC-GUCCGgaccccgaggaccucCCGGGCCC-CGAGGACc -3' miRNA: 3'- -UUGGcCAGGU---------------GGUCCGGGaGUUCCUG- -5' |
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30590 | 3' | -60 | NC_006549.1 | + | 6613 | 0.7 | 0.428745 |
Target: 5'- uACCGGgucCCACUGGGCCg-CAGGGAg -3' miRNA: 3'- uUGGCCa--GGUGGUCCGGgaGUUCCUg -5' |
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30590 | 3' | -60 | NC_006549.1 | + | 41830 | 0.71 | 0.419969 |
Target: 5'- -cCCGGgggCGCCGGGCCCgaGAGGGCc -3' miRNA: 3'- uuGGCCag-GUGGUCCGGGagUUCCUG- -5' |
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30590 | 3' | -60 | NC_006549.1 | + | 41603 | 0.71 | 0.419969 |
Target: 5'- cACCGGgaCCACCGGGaauucccgguCCC-CAAGGACu -3' miRNA: 3'- uUGGCCa-GGUGGUCC----------GGGaGUUCCUG- -5' |
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30590 | 3' | -60 | NC_006549.1 | + | 7761 | 0.71 | 0.419969 |
Target: 5'- -cUCGGggCCGCCAGGCgUaCAAGGACc -3' miRNA: 3'- uuGGCCa-GGUGGUCCGgGaGUUCCUG- -5' |
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30590 | 3' | -60 | NC_006549.1 | + | 123151 | 0.71 | 0.377773 |
Target: 5'- -uCUGGUCCGCCucuguuGCCUUCGAGGAa -3' miRNA: 3'- uuGGCCAGGUGGuc----CGGGAGUUCCUg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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