Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30590 | 5' | -59.7 | NC_006549.1 | + | 50373 | 0.67 | 0.618067 |
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Target: 5'- -uGGCCCgGGAGGACCCucUGGGCc--- -3' miRNA: 3'- acCCGGG-UUUCCUGGGu-GCCCGcuuu -5' |
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| 30590 | 5' | -59.7 | NC_006549.1 | + | 50473 | 0.67 | 0.628234 |
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Target: 5'- cGGGgCCAAcgaggccaauGGGACCCACuGGCc--- -3' miRNA: 3'- aCCCgGGUU----------UCCUGGGUGcCCGcuuu -5' |
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| 30590 | 5' | -59.7 | NC_006549.1 | + | 49679 | 0.67 | 0.638403 |
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Target: 5'- cUGGGaCCUugcGGACCCACGGuGCc--- -3' miRNA: 3'- -ACCC-GGGuuuCCUGGGUGCC-CGcuuu -5' |
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| 30590 | 5' | -59.7 | NC_006549.1 | + | 102595 | 0.67 | 0.638403 |
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Target: 5'- gGGuGCCCgGGAGGACCUcuCGGGCc--- -3' miRNA: 3'- aCC-CGGG-UUUCCUGGGu-GCCCGcuuu -5' |
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| 30590 | 5' | -59.7 | NC_006549.1 | + | 102472 | 0.67 | 0.648568 |
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Target: 5'- -uGGCCU--GGGACuCCugGGGCGc-- -3' miRNA: 3'- acCCGGGuuUCCUG-GGugCCCGCuuu -5' |
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| 30590 | 5' | -59.7 | NC_006549.1 | + | 59010 | 0.67 | 0.65872 |
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Target: 5'- gGGGCgaAGAGGGCUCcUGGGUGAu- -3' miRNA: 3'- aCCCGggUUUCCUGGGuGCCCGCUuu -5' |
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| 30590 | 5' | -59.7 | NC_006549.1 | + | 7516 | 0.67 | 0.65872 |
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Target: 5'- aGGGgCCAgagggagaccAAGGACCCAUagGGGCcAAGg -3' miRNA: 3'- aCCCgGGU----------UUCCUGGGUG--CCCGcUUU- -5' |
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| 30590 | 5' | -59.7 | NC_006549.1 | + | 76101 | 0.66 | 0.71889 |
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Target: 5'- cGGuGUUUAGAGGAUCCagcACGGGCGc-- -3' miRNA: 3'- aCC-CGGGUUUCCUGGG---UGCCCGCuuu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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