Results 1 - 20 of 32 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30594 | 5' | -58.5 | NC_006549.1 | + | 15050 | 0.66 | 0.785609 |
Target: 5'- uAUCCUAGGGucuCCUGUuucUGuGGCCGucacgaacGCCu -3' miRNA: 3'- uUGGGAUCCCu--GGGCA---AC-CCGGU--------UGG- -5' |
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30594 | 5' | -58.5 | NC_006549.1 | + | 50533 | 0.66 | 0.767094 |
Target: 5'- -gUCCgacGGGGCCCGUuuguccggUGGGuccagguggaCCAGCCg -3' miRNA: 3'- uuGGGau-CCCUGGGCA--------ACCC----------GGUUGG- -5' |
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30594 | 5' | -58.5 | NC_006549.1 | + | 102465 | 0.67 | 0.715928 |
Target: 5'- gGACuCCUGGGGcGCCaGcUGGGCCGggggguccaggaggGCCg -3' miRNA: 3'- -UUG-GGAUCCC-UGGgCaACCCGGU--------------UGG- -5' |
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30594 | 5' | -58.5 | NC_006549.1 | + | 7533 | 0.67 | 0.708 |
Target: 5'- uAGCCC-GGuGGGCCCucagGGGCCAgagggagACCa -3' miRNA: 3'- -UUGGGaUC-CCUGGGcaa-CCCGGU-------UGG- -5' |
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30594 | 5' | -58.5 | NC_006549.1 | + | 41001 | 0.68 | 0.677943 |
Target: 5'- aGGCCCccAGGGAUCCcuggGGGCCcccggucccaaugGACCg -3' miRNA: 3'- -UUGGGa-UCCCUGGGcaa-CCCGG-------------UUGG- -5' |
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30594 | 5' | -58.5 | NC_006549.1 | + | 41818 | 0.68 | 0.66885 |
Target: 5'- gGGCCCgAGaGGGCCCGc-GGGuCCAuCCg -3' miRNA: 3'- -UUGGGaUC-CCUGGGCaaCCC-GGUuGG- -5' |
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30594 | 5' | -58.5 | NC_006549.1 | + | 40938 | 0.68 | 0.638403 |
Target: 5'- gGGCCCcccGGGCCCGaugGGGCCGAg- -3' miRNA: 3'- -UUGGGaucCCUGGGCaa-CCCGGUUgg -5' |
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30594 | 5' | -58.5 | NC_006549.1 | + | 7496 | 0.69 | 0.618067 |
Target: 5'- gGACCCaUAGGGGCCaag-GGuGCCGAUg -3' miRNA: 3'- -UUGGG-AUCCCUGGgcaaCC-CGGUUGg -5' |
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30594 | 5' | -58.5 | NC_006549.1 | + | 7937 | 0.69 | 0.618067 |
Target: 5'- gGACCCgaUGGGGAgCCG--GGGCCugagGGCCc -3' miRNA: 3'- -UUGGG--AUCCCUgGGCaaCCCGG----UUGG- -5' |
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30594 | 5' | -58.5 | NC_006549.1 | + | 7386 | 0.69 | 0.618067 |
Target: 5'- gGGCCCcAGGGcccGCCCGgcccuccaGGGCCccaagGACCa -3' miRNA: 3'- -UUGGGaUCCC---UGGGCaa------CCCGG-----UUGG- -5' |
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30594 | 5' | -58.5 | NC_006549.1 | + | 50853 | 0.69 | 0.607911 |
Target: 5'- gGACCCaGGuGGGCCC-UUGGGUccuggCAGCCc -3' miRNA: 3'- -UUGGGaUC-CCUGGGcAACCCG-----GUUGG- -5' |
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30594 | 5' | -58.5 | NC_006549.1 | + | 102130 | 0.69 | 0.607911 |
Target: 5'- -gUCCUGGGGGgccaggagguCCUGggGGGCCAggauuuauACCa -3' miRNA: 3'- uuGGGAUCCCU----------GGGCaaCCCGGU--------UGG- -5' |
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30594 | 5' | -58.5 | NC_006549.1 | + | 6774 | 0.69 | 0.577578 |
Target: 5'- aAGCCCgguuaAGGGACCCGc-GGGUCcGCa -3' miRNA: 3'- -UUGGGa----UCCCUGGGCaaCCCGGuUGg -5' |
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30594 | 5' | -58.5 | NC_006549.1 | + | 51008 | 0.69 | 0.577578 |
Target: 5'- gGGCCCc-GGGGCCCcUUaGGGCCGAUn -3' miRNA: 3'- -UUGGGauCCCUGGGcAA-CCCGGUUGg -5' |
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30594 | 5' | -58.5 | NC_006549.1 | + | 7071 | 0.7 | 0.567535 |
Target: 5'- -uCCCaccGGACCCGccGGGCCcGCCg -3' miRNA: 3'- uuGGGaucCCUGGGCaaCCCGGuUGG- -5' |
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30594 | 5' | -58.5 | NC_006549.1 | + | 101855 | 0.7 | 0.547589 |
Target: 5'- gGACCCUGaGG-CCCGggaGGGCCuauagGACCg -3' miRNA: 3'- -UUGGGAUcCCuGGGCaa-CCCGG-----UUGG- -5' |
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30594 | 5' | -58.5 | NC_006549.1 | + | 6653 | 0.7 | 0.52787 |
Target: 5'- gGACCCgcuGGcGAUCCGgguccucccGGGCCGACUg -3' miRNA: 3'- -UUGGGau-CC-CUGGGCaa-------CCCGGUUGG- -5' |
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30594 | 5' | -58.5 | NC_006549.1 | + | 82544 | 0.71 | 0.489281 |
Target: 5'- gGACCCauGGGGCCCGUuggcUauucuacgcagaGGGCCAGCg -3' miRNA: 3'- -UUGGGauCCCUGGGCA----A------------CCCGGUUGg -5' |
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30594 | 5' | -58.5 | NC_006549.1 | + | 41727 | 0.71 | 0.479838 |
Target: 5'- gGACCCaGGGGcGCCCGc-GGGCCccCCg -3' miRNA: 3'- -UUGGGaUCCC-UGGGCaaCCCGGuuGG- -5' |
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30594 | 5' | -58.5 | NC_006549.1 | + | 50633 | 0.71 | 0.470484 |
Target: 5'- -cCCCUAGuGGuuCCCGcUGGGCCcuGCCc -3' miRNA: 3'- uuGGGAUC-CCu-GGGCaACCCGGu-UGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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