Results 1 - 20 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30686 | 3' | -52.7 | NC_006552.1 | + | 16779 | 0.66 | 0.870582 |
Target: 5'- gACAACcgaaGCCCAGGuGCUcGGCGGacacacUGCu -3' miRNA: 3'- -UGUUGcg--UGGGUCCuUGA-CCGUU------ACG- -5' |
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30686 | 3' | -52.7 | NC_006552.1 | + | 29832 | 0.66 | 0.870582 |
Target: 5'- gGCuuGC-CGCCCGGGAuauccgagagACUGGCcg-GCg -3' miRNA: 3'- -UGu-UGcGUGGGUCCU----------UGACCGuuaCG- -5' |
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30686 | 3' | -52.7 | NC_006552.1 | + | 39619 | 0.66 | 0.870582 |
Target: 5'- cAguACGCugaGCCgCGGGAA--GGCAAUGCc -3' miRNA: 3'- -UguUGCG---UGG-GUCCUUgaCCGUUACG- -5' |
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30686 | 3' | -52.7 | NC_006552.1 | + | 53259 | 0.66 | 0.86324 |
Target: 5'- -uGGCGCGCCaggcucgcuaggucGGAGCgcaUGGCGGUGUg -3' miRNA: 3'- ugUUGCGUGGgu------------CCUUG---ACCGUUACG- -5' |
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30686 | 3' | -52.7 | NC_006552.1 | + | 44066 | 0.66 | 0.862411 |
Target: 5'- gGCuGCuCGCCCAGGuuGACgGGCGGcgGCg -3' miRNA: 3'- -UGuUGcGUGGGUCC--UUGaCCGUUa-CG- -5' |
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30686 | 3' | -52.7 | NC_006552.1 | + | 8056 | 0.66 | 0.853993 |
Target: 5'- gACAACcaGCA-CCGGGAcCUGGCg--GCg -3' miRNA: 3'- -UGUUG--CGUgGGUCCUuGACCGuuaCG- -5' |
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30686 | 3' | -52.7 | NC_006552.1 | + | 57739 | 0.66 | 0.853993 |
Target: 5'- cGCGACGgACCCgccgAGGuACUGcGCAAacaGCa -3' miRNA: 3'- -UGUUGCgUGGG----UCCuUGAC-CGUUa--CG- -5' |
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30686 | 3' | -52.7 | NC_006552.1 | + | 24608 | 0.66 | 0.853993 |
Target: 5'- uACcACGCACgcugguCCGGGucgaUGGUGAUGCg -3' miRNA: 3'- -UGuUGCGUG------GGUCCuug-ACCGUUACG- -5' |
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30686 | 3' | -52.7 | NC_006552.1 | + | 2058 | 0.66 | 0.836445 |
Target: 5'- cGCGGCGaaCACCCGcacaccgcGGAGCaGGCGAaccUGCc -3' miRNA: 3'- -UGUUGC--GUGGGU--------CCUUGaCCGUU---ACG- -5' |
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30686 | 3' | -52.7 | NC_006552.1 | + | 46404 | 0.67 | 0.827334 |
Target: 5'- -gAACGUGCCUGGGcgUUGGCGGUa- -3' miRNA: 3'- ugUUGCGUGGGUCCuuGACCGUUAcg -5' |
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30686 | 3' | -52.7 | NC_006552.1 | + | 53069 | 0.67 | 0.818011 |
Target: 5'- ---cCGCACgguguCCAGGAACUuGGCcuUGCc -3' miRNA: 3'- uguuGCGUG-----GGUCCUUGA-CCGuuACG- -5' |
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30686 | 3' | -52.7 | NC_006552.1 | + | 8277 | 0.67 | 0.788875 |
Target: 5'- ---cCGCGCCCGcgcuGGACUGGCGcaacuaccccgAUGCg -3' miRNA: 3'- uguuGCGUGGGUc---CUUGACCGU-----------UACG- -5' |
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30686 | 3' | -52.7 | NC_006552.1 | + | 25956 | 0.67 | 0.788875 |
Target: 5'- gGC-AUGC-UCUGGGAACaccagGGCAAUGCg -3' miRNA: 3'- -UGuUGCGuGGGUCCUUGa----CCGUUACG- -5' |
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30686 | 3' | -52.7 | NC_006552.1 | + | 12812 | 0.68 | 0.758225 |
Target: 5'- aGCAuCGCGCCUcauuGGGcGCaGGCAGUGg -3' miRNA: 3'- -UGUuGCGUGGG----UCCuUGaCCGUUACg -5' |
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30686 | 3' | -52.7 | NC_006552.1 | + | 19817 | 0.69 | 0.726377 |
Target: 5'- gGCAGCGCgGCCCGGGcuCUuGCucgGCg -3' miRNA: 3'- -UGUUGCG-UGGGUCCuuGAcCGuuaCG- -5' |
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30686 | 3' | -52.7 | NC_006552.1 | + | 31683 | 0.69 | 0.715551 |
Target: 5'- cGCGAacaGCGCCUugcuGGAACUGGUgc-GCa -3' miRNA: 3'- -UGUUg--CGUGGGu---CCUUGACCGuuaCG- -5' |
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30686 | 3' | -52.7 | NC_006552.1 | + | 3604 | 0.69 | 0.715551 |
Target: 5'- cGCAucuACGuCGCCuacgaCAGGGACUGGgAcgGCa -3' miRNA: 3'- -UGU---UGC-GUGG-----GUCCUUGACCgUuaCG- -5' |
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30686 | 3' | -52.7 | NC_006552.1 | + | 16679 | 0.69 | 0.693655 |
Target: 5'- --cGCGCGCCCAGcauccuGAACggaGGCAcacAUGCu -3' miRNA: 3'- uguUGCGUGGGUC------CUUGa--CCGU---UACG- -5' |
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30686 | 3' | -52.7 | NC_006552.1 | + | 43172 | 0.7 | 0.638044 |
Target: 5'- gGCGGCGUccucgaCCAGGAcACUGGCGgccuggccaGUGCu -3' miRNA: 3'- -UGUUGCGug----GGUCCU-UGACCGU---------UACG- -5' |
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30686 | 3' | -52.7 | NC_006552.1 | + | 56581 | 0.7 | 0.638044 |
Target: 5'- uCAGCGCcCaCCGGGGACUuuGCAAUGUc -3' miRNA: 3'- uGUUGCGuG-GGUCCUUGAc-CGUUACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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