Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30686 | 3' | -52.7 | NC_006552.1 | + | 32723 | 0.7 | 0.626861 |
Target: 5'- cAUAcCGCGCCaccGAACUGGuCGAUGCg -3' miRNA: 3'- -UGUuGCGUGGgucCUUGACC-GUUACG- -5' |
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30686 | 3' | -52.7 | NC_006552.1 | + | 59539 | 0.71 | 0.604521 |
Target: 5'- cGCAACGC-CCCcGGA--UGGCcucGAUGCg -3' miRNA: 3'- -UGUUGCGuGGGuCCUugACCG---UUACG- -5' |
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30686 | 3' | -52.7 | NC_006552.1 | + | 52251 | 0.71 | 0.593383 |
Target: 5'- cCAGCGCgcugGCCUuGGc-CUGGCGGUGCg -3' miRNA: 3'- uGUUGCG----UGGGuCCuuGACCGUUACG- -5' |
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30686 | 3' | -52.7 | NC_006552.1 | + | 4308 | 0.72 | 0.538373 |
Target: 5'- uGCGGCGCACC--GGcGCUGGCAuaucggGCg -3' miRNA: 3'- -UGUUGCGUGGguCCuUGACCGUua----CG- -5' |
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30686 | 3' | -52.7 | NC_006552.1 | + | 8958 | 0.72 | 0.538373 |
Target: 5'- gGCuACGCACCCAccgucgcaGAACUGGCAGccaagGCc -3' miRNA: 3'- -UGuUGCGUGGGUc-------CUUGACCGUUa----CG- -5' |
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30686 | 3' | -52.7 | NC_006552.1 | + | 1505 | 0.76 | 0.327022 |
Target: 5'- cCGACGCaagccGCCCAGGAcCUGGCAcuccGCa -3' miRNA: 3'- uGUUGCG-----UGGGUCCUuGACCGUua--CG- -5' |
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30686 | 3' | -52.7 | NC_006552.1 | + | 1305 | 1.12 | 0.001266 |
Target: 5'- gACAACGCACCCAGGAACUGGCAAUGCu -3' miRNA: 3'- -UGUUGCGUGGGUCCUUGACCGUUACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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