Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30686 | 3' | -52.7 | NC_006552.1 | + | 57739 | 0.66 | 0.853993 |
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Target: 5'- cGCGACGgACCCgccgAGGuACUGcGCAAacaGCa -3' miRNA: 3'- -UGUUGCgUGGG----UCCuUGAC-CGUUa--CG- -5' |
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| 30686 | 3' | -52.7 | NC_006552.1 | + | 24608 | 0.66 | 0.853993 |
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Target: 5'- uACcACGCACgcugguCCGGGucgaUGGUGAUGCg -3' miRNA: 3'- -UGuUGCGUG------GGUCCuug-ACCGUUACG- -5' |
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| 30686 | 3' | -52.7 | NC_006552.1 | + | 44066 | 0.66 | 0.862411 |
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Target: 5'- gGCuGCuCGCCCAGGuuGACgGGCGGcgGCg -3' miRNA: 3'- -UGuUGcGUGGGUCC--UUGaCCGUUa-CG- -5' |
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| 30686 | 3' | -52.7 | NC_006552.1 | + | 53259 | 0.66 | 0.86324 |
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Target: 5'- -uGGCGCGCCaggcucgcuaggucGGAGCgcaUGGCGGUGUg -3' miRNA: 3'- ugUUGCGUGGgu------------CCUUG---ACCGUUACG- -5' |
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| 30686 | 3' | -52.7 | NC_006552.1 | + | 39619 | 0.66 | 0.870582 |
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Target: 5'- cAguACGCugaGCCgCGGGAA--GGCAAUGCc -3' miRNA: 3'- -UguUGCG---UGG-GUCCUUgaCCGUUACG- -5' |
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| 30686 | 3' | -52.7 | NC_006552.1 | + | 29832 | 0.66 | 0.870582 |
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Target: 5'- gGCuuGC-CGCCCGGGAuauccgagagACUGGCcg-GCg -3' miRNA: 3'- -UGu-UGcGUGGGUCCU----------UGACCGuuaCG- -5' |
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| 30686 | 3' | -52.7 | NC_006552.1 | + | 16779 | 0.66 | 0.870582 |
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Target: 5'- gACAACcgaaGCCCAGGuGCUcGGCGGacacacUGCu -3' miRNA: 3'- -UGUUGcg--UGGGUCCuUGA-CCGUU------ACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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