Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 30686 | 3' | -52.7 | NC_006552.1 | + | 32723 | 0.7 | 0.626861 |
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Target: 5'- cAUAcCGCGCCaccGAACUGGuCGAUGCg -3' miRNA: 3'- -UGUuGCGUGGgucCUUGACC-GUUACG- -5' |
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| 30686 | 3' | -52.7 | NC_006552.1 | + | 56581 | 0.7 | 0.638044 |
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Target: 5'- uCAGCGCcCaCCGGGGACUuuGCAAUGUc -3' miRNA: 3'- uGUUGCGuG-GGUCCUUGAc-CGUUACG- -5' |
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| 30686 | 3' | -52.7 | NC_006552.1 | + | 43172 | 0.7 | 0.638044 |
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Target: 5'- gGCGGCGUccucgaCCAGGAcACUGGCGgccuggccaGUGCu -3' miRNA: 3'- -UGUUGCGug----GGUCCU-UGACCGU---------UACG- -5' |
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| 30686 | 3' | -52.7 | NC_006552.1 | + | 16679 | 0.69 | 0.693655 |
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Target: 5'- --cGCGCGCCCAGcauccuGAACggaGGCAcacAUGCu -3' miRNA: 3'- uguUGCGUGGGUC------CUUGa--CCGU---UACG- -5' |
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| 30686 | 3' | -52.7 | NC_006552.1 | + | 3604 | 0.69 | 0.715551 |
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Target: 5'- cGCAucuACGuCGCCuacgaCAGGGACUGGgAcgGCa -3' miRNA: 3'- -UGU---UGC-GUGG-----GUCCUUGACCgUuaCG- -5' |
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| 30686 | 3' | -52.7 | NC_006552.1 | + | 31683 | 0.69 | 0.715551 |
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Target: 5'- cGCGAacaGCGCCUugcuGGAACUGGUgc-GCa -3' miRNA: 3'- -UGUUg--CGUGGGu---CCUUGACCGuuaCG- -5' |
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| 30686 | 3' | -52.7 | NC_006552.1 | + | 16779 | 0.66 | 0.870582 |
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Target: 5'- gACAACcgaaGCCCAGGuGCUcGGCGGacacacUGCu -3' miRNA: 3'- -UGUUGcg--UGGGUCCuUGA-CCGUU------ACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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