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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30688 | 3' | -56.5 | NC_006552.1 | + | 4639 | 0.66 | 0.658368 |
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Target: 5'- cGCCGGCGAcucgccgcaggcaUGUcccucgaGGAcGCGCUGCu- -3' miRNA: 3'- aCGGCUGCU-------------ACAag-----CCU-CGCGACGuu -5' |
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| 30688 | 3' | -56.5 | NC_006552.1 | + | 49647 | 0.67 | 0.615659 |
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Target: 5'- cGCCGAU--UGgcgUCGGGGCuuGCUGCu- -3' miRNA: 3'- aCGGCUGcuACa--AGCCUCG--CGACGuu -5' |
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| 30688 | 3' | -56.5 | NC_006552.1 | + | 46320 | 0.67 | 0.593804 |
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Target: 5'- cGCUGACGGUGcacagCGaGAGCcuGCUGCu- -3' miRNA: 3'- aCGGCUGCUACaa---GC-CUCG--CGACGuu -5' |
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| 30688 | 3' | -56.5 | NC_006552.1 | + | 49569 | 0.67 | 0.582921 |
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Target: 5'- gGCCaGCGgcGUUCuGGuGGCGCUGCu- -3' miRNA: 3'- aCGGcUGCuaCAAG-CC-UCGCGACGuu -5' |
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| 30688 | 3' | -56.5 | NC_006552.1 | + | 556 | 0.68 | 0.518745 |
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Target: 5'- gGCCGACGcUGUUCGccGCGCcGCc- -3' miRNA: 3'- aCGGCUGCuACAAGCcuCGCGaCGuu -5' |
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| 30688 | 3' | -56.5 | NC_006552.1 | + | 41941 | 0.69 | 0.47653 |
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Target: 5'- cGCCGACGcaccaaaGUGUcggCGGAGUGgaGUAAg -3' miRNA: 3'- aCGGCUGC-------UACAa--GCCUCGCgaCGUU- -5' |
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| 30688 | 3' | -56.5 | NC_006552.1 | + | 32490 | 0.7 | 0.428432 |
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Target: 5'- gGCCGGCGcgGggccggUGGAGCGCU-CGAa -3' miRNA: 3'- aCGGCUGCuaCaa----GCCUCGCGAcGUU- -5' |
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| 30688 | 3' | -56.5 | NC_006552.1 | + | 2022 | 1.06 | 0.001246 |
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Target: 5'- cUGCCGACGAUGUUCGGAGCGCUGCAAg -3' miRNA: 3'- -ACGGCUGCUACAAGCCUCGCGACGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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