Results 1 - 20 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30688 | 5' | -59.2 | NC_006552.1 | + | 43330 | 0.66 | 0.587044 |
Target: 5'- cGCCGCcaGAUGGUccAGCaCCagCGCGGCAa -3' miRNA: 3'- cCGGCG--UUGCCAc-UUG-GGa-GCGCCGU- -5' |
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30688 | 5' | -59.2 | NC_006552.1 | + | 49995 | 0.66 | 0.587044 |
Target: 5'- cGGCCuuGAUGGUGccgguCgCCUUGCGGUu -3' miRNA: 3'- -CCGGcgUUGCCACuu---G-GGAGCGCCGu -5' |
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30688 | 5' | -59.2 | NC_006552.1 | + | 43386 | 0.66 | 0.585995 |
Target: 5'- uGGUCGCcccccACGGUGAuguCCUCGaucaccaCGGCAu -3' miRNA: 3'- -CCGGCGu----UGCCACUug-GGAGC-------GCCGU- -5' |
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30688 | 5' | -59.2 | NC_006552.1 | + | 2818 | 0.66 | 0.57553 |
Target: 5'- uGGaCCGCAACuGUucgacugGAACCC-CGCGGa- -3' miRNA: 3'- -CC-GGCGUUGcCA-------CUUGGGaGCGCCgu -5' |
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30688 | 5' | -59.2 | NC_006552.1 | + | 5129 | 0.66 | 0.555769 |
Target: 5'- aGGCCuGCGGCGGUcGGGCCaCUCuccaGCaGCu -3' miRNA: 3'- -CCGG-CGUUGCCA-CUUGG-GAG----CGcCGu -5' |
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30688 | 5' | -59.2 | NC_006552.1 | + | 28992 | 0.66 | 0.535187 |
Target: 5'- cGGCUGCGACGuUGGGCUCgUCGCuGUc -3' miRNA: 3'- -CCGGCGUUGCcACUUGGG-AGCGcCGu -5' |
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30688 | 5' | -59.2 | NC_006552.1 | + | 35983 | 0.66 | 0.535187 |
Target: 5'- uGGCUGauGCGGUGGACauccuggaUCGCGGUc -3' miRNA: 3'- -CCGGCguUGCCACUUGgg------AGCGCCGu -5' |
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30688 | 5' | -59.2 | NC_006552.1 | + | 2091 | 0.66 | 0.534165 |
Target: 5'- uGCCGUgcGAUGGUGGaguugAUCCUCaucgagcGCGGCGa -3' miRNA: 3'- cCGGCG--UUGCCACU-----UGGGAG-------CGCCGU- -5' |
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30688 | 5' | -59.2 | NC_006552.1 | + | 36588 | 0.67 | 0.524996 |
Target: 5'- aGGauCgGCGGCGGUucGAugCCUUccaGCGGCAg -3' miRNA: 3'- -CC--GgCGUUGCCA--CUugGGAG---CGCCGU- -5' |
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30688 | 5' | -59.2 | NC_006552.1 | + | 3207 | 0.67 | 0.51488 |
Target: 5'- uGCaGCAGCGaGgccGAACUCUgCGCGGCGu -3' miRNA: 3'- cCGgCGUUGC-Ca--CUUGGGA-GCGCCGU- -5' |
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30688 | 5' | -59.2 | NC_006552.1 | + | 31826 | 0.67 | 0.51488 |
Target: 5'- gGGCCaGC-ACGGgccGGCCCUUcguaggGCGGCGg -3' miRNA: 3'- -CCGG-CGuUGCCac-UUGGGAG------CGCCGU- -5' |
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30688 | 5' | -59.2 | NC_006552.1 | + | 33191 | 0.68 | 0.465598 |
Target: 5'- cGGCUGC-GCGGUG--UCCUUGuCGGCc -3' miRNA: 3'- -CCGGCGuUGCCACuuGGGAGC-GCCGu -5' |
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30688 | 5' | -59.2 | NC_006552.1 | + | 36530 | 0.68 | 0.465598 |
Target: 5'- uGGCUGCAGUGGUG-AUCUgcgGCGGCGc -3' miRNA: 3'- -CCGGCGUUGCCACuUGGGag-CGCCGU- -5' |
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30688 | 5' | -59.2 | NC_006552.1 | + | 53071 | 0.68 | 0.459847 |
Target: 5'- uGCCGC-ACGGUGuccaGgaacuuggccuugccGCCCUCGaUGGCAu -3' miRNA: 3'- cCGGCGuUGCCAC----U---------------UGGGAGC-GCCGU- -5' |
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30688 | 5' | -59.2 | NC_006552.1 | + | 56050 | 0.68 | 0.446577 |
Target: 5'- uGCCGCcgucacccuGCGcGU-AGCCgCUCGCGGCAu -3' miRNA: 3'- cCGGCGu--------UGC-CAcUUGG-GAGCGCCGU- -5' |
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30688 | 5' | -59.2 | NC_006552.1 | + | 45636 | 0.68 | 0.427996 |
Target: 5'- cGGCCGCuagcugAGCGGUGuacugccccagGGCCUUCGCcGCc -3' miRNA: 3'- -CCGGCG------UUGCCAC-----------UUGGGAGCGcCGu -5' |
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30688 | 5' | -59.2 | NC_006552.1 | + | 21318 | 0.68 | 0.427996 |
Target: 5'- uGGCC-CAuGCGGUGGACCag-GCGGUg -3' miRNA: 3'- -CCGGcGU-UGCCACUUGGgagCGCCGu -5' |
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30688 | 5' | -59.2 | NC_006552.1 | + | 20398 | 0.68 | 0.417973 |
Target: 5'- aGCgGCAACcGUGAaucguccGCCCUCcCGGCGa -3' miRNA: 3'- cCGgCGUUGcCACU-------UGGGAGcGCCGU- -5' |
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30688 | 5' | -59.2 | NC_006552.1 | + | 7689 | 0.69 | 0.39225 |
Target: 5'- gGGCCGCc-CGGUGAA----CGCGGCAa -3' miRNA: 3'- -CCGGCGuuGCCACUUgggaGCGCCGU- -5' |
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30688 | 5' | -59.2 | NC_006552.1 | + | 57177 | 0.7 | 0.326841 |
Target: 5'- aGCgCGaCAGCGGUGcauAGCCCUCGCccuGCAa -3' miRNA: 3'- cCG-GC-GUUGCCAC---UUGGGAGCGc--CGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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