Results 1 - 20 of 28 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30690 | 3' | -60 | NC_006552.1 | + | 45456 | 0.66 | 0.516382 |
Target: 5'- -uGGCGCUGGcCGcu-GCGCgCCAGgCu -3' miRNA: 3'- guCCGCGACCuGUucuCGCG-GGUCgG- -5' |
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30690 | 3' | -60 | NC_006552.1 | + | 38662 | 0.66 | 0.506263 |
Target: 5'- -cGGCGCcGGgguguccaGCGucaGGCGCUCGGCCu -3' miRNA: 3'- guCCGCGaCC--------UGUuc-UCGCGGGUCGG- -5' |
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30690 | 3' | -60 | NC_006552.1 | + | 27434 | 0.66 | 0.496231 |
Target: 5'- uCGGGCGCUGGuaucccGCGCUCuGCa -3' miRNA: 3'- -GUCCGCGACCuguucuCGCGGGuCGg -5' |
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30690 | 3' | -60 | NC_006552.1 | + | 50714 | 0.66 | 0.496231 |
Target: 5'- cCAGGuCGC-GGAUggGGGCaUCCAGgCg -3' miRNA: 3'- -GUCC-GCGaCCUGuuCUCGcGGGUCgG- -5' |
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30690 | 3' | -60 | NC_006552.1 | + | 23807 | 0.66 | 0.496231 |
Target: 5'- aCAGGCGCcacucagGGucagcCAGGcAGCGCgCCAGUUc -3' miRNA: 3'- -GUCCGCGa------CCu----GUUC-UCGCG-GGUCGG- -5' |
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30690 | 3' | -60 | NC_006552.1 | + | 59385 | 0.66 | 0.476443 |
Target: 5'- --cGCGCcc-GCAAGGGCGCC-AGCCa -3' miRNA: 3'- gucCGCGaccUGUUCUCGCGGgUCGG- -5' |
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30690 | 3' | -60 | NC_006552.1 | + | 48389 | 0.66 | 0.476443 |
Target: 5'- aCAGGUgccaacGCUGGAgcc-AGCGCCCGaacGCCa -3' miRNA: 3'- -GUCCG------CGACCUguucUCGCGGGU---CGG- -5' |
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30690 | 3' | -60 | NC_006552.1 | + | 2606 | 0.66 | 0.466696 |
Target: 5'- ----gGCUcGACAAGGGCGCCgAGCg -3' miRNA: 3'- guccgCGAcCUGUUCUCGCGGgUCGg -5' |
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30690 | 3' | -60 | NC_006552.1 | + | 58043 | 0.67 | 0.457054 |
Target: 5'- aCAGGCGCgccacgcuguUGGACGcaAGUGCUCgAGCa -3' miRNA: 3'- -GUCCGCG----------ACCUGUucUCGCGGG-UCGg -5' |
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30690 | 3' | -60 | NC_006552.1 | + | 21454 | 0.67 | 0.44752 |
Target: 5'- gAGGCGC--GACAc--GCGcCCCAGCCc -3' miRNA: 3'- gUCCGCGacCUGUucuCGC-GGGUCGG- -5' |
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30690 | 3' | -60 | NC_006552.1 | + | 34375 | 0.67 | 0.44752 |
Target: 5'- -uGGUGCcacUGGGCAu-AGCGCuCCAGCa -3' miRNA: 3'- guCCGCG---ACCUGUucUCGCG-GGUCGg -5' |
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30690 | 3' | -60 | NC_006552.1 | + | 22011 | 0.67 | 0.410528 |
Target: 5'- cCAGGUGCUGGuCGuaGGCcuGCUCGGUCu -3' miRNA: 3'- -GUCCGCGACCuGUucUCG--CGGGUCGG- -5' |
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30690 | 3' | -60 | NC_006552.1 | + | 12222 | 0.67 | 0.410528 |
Target: 5'- cCGGGUGCgcguuCAucGAGCGCgCAGCCu -3' miRNA: 3'- -GUCCGCGaccu-GUu-CUCGCGgGUCGG- -5' |
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30690 | 3' | -60 | NC_006552.1 | + | 63146 | 0.67 | 0.410528 |
Target: 5'- aCAGGCGCUu-----GAGCGCgCCGGCg -3' miRNA: 3'- -GUCCGCGAccuguuCUCGCG-GGUCGg -5' |
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30690 | 3' | -60 | NC_006552.1 | + | 16195 | 0.68 | 0.375496 |
Target: 5'- cCGGGCaaGCccgGcGGCGcGAGCGUUCAGCCg -3' miRNA: 3'- -GUCCG--CGa--C-CUGUuCUCGCGGGUCGG- -5' |
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30690 | 3' | -60 | NC_006552.1 | + | 50891 | 0.68 | 0.367059 |
Target: 5'- -cGGCGC-GGGCcuGGGC-CUCGGCCu -3' miRNA: 3'- guCCGCGaCCUGuuCUCGcGGGUCGG- -5' |
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30690 | 3' | -60 | NC_006552.1 | + | 2128 | 0.68 | 0.367059 |
Target: 5'- cCAGGUGCUGGGCAuGGccaaCGCCgucGCCg -3' miRNA: 3'- -GUCCGCGACCUGUuCUc---GCGGgu-CGG- -5' |
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30690 | 3' | -60 | NC_006552.1 | + | 31776 | 0.69 | 0.318469 |
Target: 5'- gAGGCGCucgccgUGGACGaucaccuGGAGCuuGCCC-GCCu -3' miRNA: 3'- gUCCGCG------ACCUGU-------UCUCG--CGGGuCGG- -5' |
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30690 | 3' | -60 | NC_006552.1 | + | 32487 | 0.69 | 0.311723 |
Target: 5'- -cGGCGCgGGGCcgguGGAGCGCUCgaacaGGUCg -3' miRNA: 3'- guCCGCGaCCUGu---UCUCGCGGG-----UCGG- -5' |
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30690 | 3' | -60 | NC_006552.1 | + | 32696 | 0.71 | 0.25652 |
Target: 5'- --cGCGCgUGGACGgcaucacgGGAGCGCgCCaAGCCg -3' miRNA: 3'- gucCGCG-ACCUGU--------UCUCGCG-GG-UCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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