Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 30690 | 3' | -60 | NC_006552.1 | + | 47551 | 0.72 | 0.206379 |
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Target: 5'- cCAGGCGCUGGcgaucgaguucguugGCGgcgaGGAagcgcGCGCCCuGCCc -3' miRNA: 3'- -GUCCGCGACC---------------UGU----UCU-----CGCGGGuCGG- -5' |
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| 30690 | 3' | -60 | NC_006552.1 | + | 48389 | 0.66 | 0.476443 |
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Target: 5'- aCAGGUgccaacGCUGGAgcc-AGCGCCCGaacGCCa -3' miRNA: 3'- -GUCCG------CGACCUguucUCGCGGGU---CGG- -5' |
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| 30690 | 3' | -60 | NC_006552.1 | + | 50714 | 0.66 | 0.496231 |
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Target: 5'- cCAGGuCGC-GGAUggGGGCaUCCAGgCg -3' miRNA: 3'- -GUCC-GCGaCCUGuuCUCGcGGGUCgG- -5' |
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| 30690 | 3' | -60 | NC_006552.1 | + | 50891 | 0.68 | 0.367059 |
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Target: 5'- -cGGCGC-GGGCcuGGGC-CUCGGCCu -3' miRNA: 3'- guCCGCGaCCUGuuCUCGcGGGUCGG- -5' |
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| 30690 | 3' | -60 | NC_006552.1 | + | 58043 | 0.67 | 0.457054 |
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Target: 5'- aCAGGCGCgccacgcuguUGGACGcaAGUGCUCgAGCa -3' miRNA: 3'- -GUCCGCG----------ACCUGUucUCGCGGG-UCGg -5' |
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| 30690 | 3' | -60 | NC_006552.1 | + | 59385 | 0.66 | 0.476443 |
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Target: 5'- --cGCGCcc-GCAAGGGCGCC-AGCCa -3' miRNA: 3'- gucCGCGaccUGUUCUCGCGGgUCGG- -5' |
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| 30690 | 3' | -60 | NC_006552.1 | + | 61624 | 0.71 | 0.250211 |
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Target: 5'- cCAGGUGCUGGcCGAGccgccGGCG-CCGGCa -3' miRNA: 3'- -GUCCGCGACCuGUUC-----UCGCgGGUCGg -5' |
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| 30690 | 3' | -60 | NC_006552.1 | + | 63146 | 0.67 | 0.410528 |
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Target: 5'- aCAGGCGCUu-----GAGCGCgCCGGCg -3' miRNA: 3'- -GUCCGCGAccuguuCUCGCG-GGUCGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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