Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30690 | 3' | -60 | NC_006552.1 | + | 2606 | 0.66 | 0.466696 |
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Target: 5'- ----gGCUcGACAAGGGCGCCgAGCg -3' miRNA: 3'- guccgCGAcCUGUUCUCGCGGgUCGg -5' |
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| 30690 | 3' | -60 | NC_006552.1 | + | 59385 | 0.66 | 0.476443 |
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Target: 5'- --cGCGCcc-GCAAGGGCGCC-AGCCa -3' miRNA: 3'- gucCGCGaccUGUUCUCGCGGgUCGG- -5' |
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| 30690 | 3' | -60 | NC_006552.1 | + | 48389 | 0.66 | 0.476443 |
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Target: 5'- aCAGGUgccaacGCUGGAgcc-AGCGCCCGaacGCCa -3' miRNA: 3'- -GUCCG------CGACCUguucUCGCGGGU---CGG- -5' |
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| 30690 | 3' | -60 | NC_006552.1 | + | 23807 | 0.66 | 0.496231 |
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Target: 5'- aCAGGCGCcacucagGGucagcCAGGcAGCGCgCCAGUUc -3' miRNA: 3'- -GUCCGCGa------CCu----GUUC-UCGCG-GGUCGG- -5' |
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| 30690 | 3' | -60 | NC_006552.1 | + | 50714 | 0.66 | 0.496231 |
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Target: 5'- cCAGGuCGC-GGAUggGGGCaUCCAGgCg -3' miRNA: 3'- -GUCC-GCGaCCUGuuCUCGcGGGUCgG- -5' |
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| 30690 | 3' | -60 | NC_006552.1 | + | 27434 | 0.66 | 0.496231 |
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Target: 5'- uCGGGCGCUGGuaucccGCGCUCuGCa -3' miRNA: 3'- -GUCCGCGACCuguucuCGCGGGuCGg -5' |
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| 30690 | 3' | -60 | NC_006552.1 | + | 38662 | 0.66 | 0.506263 |
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Target: 5'- -cGGCGCcGGgguguccaGCGucaGGCGCUCGGCCu -3' miRNA: 3'- guCCGCGaCC--------UGUuc-UCGCGGGUCGG- -5' |
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| 30690 | 3' | -60 | NC_006552.1 | + | 45456 | 0.66 | 0.516382 |
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Target: 5'- -uGGCGCUGGcCGcu-GCGCgCCAGgCu -3' miRNA: 3'- guCCGCGACCuGUucuCGCG-GGUCgG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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