Results 1 - 10 of 10 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30692 | 3' | -56.3 | NC_006552.1 | + | 30848 | 0.66 | 0.674178 |
Target: 5'- -aCGCGGAagAUCGgGUGC-UGCUUGGc -3' miRNA: 3'- cgGUGCCU--UAGCgCGCGgACGAACUu -5' |
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30692 | 3' | -56.3 | NC_006552.1 | + | 5224 | 0.66 | 0.652377 |
Target: 5'- cGCCcuaGCGGAuguucaccgcgAUCuGCGCGCCcUGCUguUGGGu -3' miRNA: 3'- -CGG---UGCCU-----------UAG-CGCGCGG-ACGA--ACUU- -5' |
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30692 | 3' | -56.3 | NC_006552.1 | + | 40630 | 0.66 | 0.651285 |
Target: 5'- aGCCAUGGcaccgcuguaccaAGUCGgGCGU-UGCUUGAc -3' miRNA: 3'- -CGGUGCC-------------UUAGCgCGCGgACGAACUu -5' |
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30692 | 3' | -56.3 | NC_006552.1 | + | 43356 | 0.67 | 0.608633 |
Target: 5'- aCCACGGcauccgauaccaGGUCGCGCGCC-GCcaGAu -3' miRNA: 3'- cGGUGCC------------UUAGCGCGCGGaCGaaCUu -5' |
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30692 | 3' | -56.3 | NC_006552.1 | + | 48862 | 0.67 | 0.597724 |
Target: 5'- aGCCACGGGAU-GCG-GCCgGCU-GAc -3' miRNA: 3'- -CGGUGCCUUAgCGCgCGGaCGAaCUu -5' |
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30692 | 3' | -56.3 | NC_006552.1 | + | 7730 | 0.67 | 0.597724 |
Target: 5'- uGCgGCGGAggUG-GCGCCgguacggGCUUGGAa -3' miRNA: 3'- -CGgUGCCUuaGCgCGCGGa------CGAACUU- -5' |
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30692 | 3' | -56.3 | NC_006552.1 | + | 32682 | 0.7 | 0.451439 |
Target: 5'- aUCACGGGA--GCGCGCCaagccgugcUGCUUGAc -3' miRNA: 3'- cGGUGCCUUagCGCGCGG---------ACGAACUu -5' |
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30692 | 3' | -56.3 | NC_006552.1 | + | 13557 | 0.7 | 0.422586 |
Target: 5'- cGCCaggaACGG-AUCGCaCGCCUGCUgucGAAg -3' miRNA: 3'- -CGG----UGCCuUAGCGcGCGGACGAa--CUU- -5' |
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30692 | 3' | -56.3 | NC_006552.1 | + | 48061 | 0.74 | 0.242008 |
Target: 5'- gGCCACGGAuaggccaagGUCGCGCGCaUGCccGAu -3' miRNA: 3'- -CGGUGCCU---------UAGCGCGCGgACGaaCUu -5' |
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30692 | 3' | -56.3 | NC_006552.1 | + | 2952 | 1.08 | 0.000944 |
Target: 5'- aGCCACGGAAUCGCGCGCCUGCUUGAAa -3' miRNA: 3'- -CGGUGCCUUAGCGCGCGGACGAACUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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