Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30692 | 5' | -57.3 | NC_006552.1 | + | 30935 | 0.67 | 0.60079 |
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Target: 5'- --gCGCAGUACUUGauggugUCG-CCCGCGu -3' miRNA: 3'- guaGCGUCAUGAGCga----GGCaGGGCGC- -5' |
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| 30692 | 5' | -57.3 | NC_006552.1 | + | 38111 | 0.67 | 0.60079 |
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Target: 5'- aCGUUGCGG-ACUCGCaUgCGaUCCGCGa -3' miRNA: 3'- -GUAGCGUCaUGAGCG-AgGCaGGGCGC- -5' |
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| 30692 | 5' | -57.3 | NC_006552.1 | + | 21299 | 0.68 | 0.527381 |
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Target: 5'- ---gGCGGUGCUUGCgaaC-UCCCGCGg -3' miRNA: 3'- guagCGUCAUGAGCGag-GcAGGGCGC- -5' |
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| 30692 | 5' | -57.3 | NC_006552.1 | + | 48773 | 0.69 | 0.486925 |
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Target: 5'- uCAUC-CAGgccGCUCGC-CCaGUCCCGCc -3' miRNA: 3'- -GUAGcGUCa--UGAGCGaGG-CAGGGCGc -5' |
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| 30692 | 5' | -57.3 | NC_006552.1 | + | 46655 | 0.69 | 0.486925 |
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Target: 5'- --gCGCuacGUcgUCGC-CCGUCCCGCGg -3' miRNA: 3'- guaGCGu--CAugAGCGaGGCAGGGCGC- -5' |
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| 30692 | 5' | -57.3 | NC_006552.1 | + | 2918 | 1.09 | 0.000867 |
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Target: 5'- gCAUCGCAGUACUCGCUCCGUCCCGCGg -3' miRNA: 3'- -GUAGCGUCAUGAGCGAGGCAGGGCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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