Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30694 | 3' | -60.8 | NC_006552.1 | + | 51876 | 0.66 | 0.461972 |
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Target: 5'- --uGCCGGuucGgACUGGGGCCGGgCUgCa -3' miRNA: 3'- guuCGGCCu--CgUGGUCCCGGUC-GAgG- -5' |
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| 30694 | 3' | -60.8 | NC_006552.1 | + | 21207 | 0.66 | 0.461972 |
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Target: 5'- --uGCCGcugucGGCgACCAGGGCCAGgCgaaCCu -3' miRNA: 3'- guuCGGCc----UCG-UGGUCCCGGUC-Ga--GG- -5' |
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| 30694 | 3' | -60.8 | NC_006552.1 | + | 56772 | 0.66 | 0.471614 |
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Target: 5'- uCAGGCC---GCGCCAGGcGCgCAGCagUCCc -3' miRNA: 3'- -GUUCGGccuCGUGGUCC-CG-GUCG--AGG- -5' |
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| 30694 | 3' | -60.8 | NC_006552.1 | + | 31034 | 0.66 | 0.471614 |
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Target: 5'- aGAGCCuGGccgcccAGCAgCAGGGCgCGG-UCCa -3' miRNA: 3'- gUUCGG-CC------UCGUgGUCCCG-GUCgAGG- -5' |
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| 30694 | 3' | -60.8 | NC_006552.1 | + | 23381 | 0.66 | 0.475499 |
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Target: 5'- --cGCCGucgcccgcacacucuGGGCAuCCAGGGUgCGGCUCg -3' miRNA: 3'- guuCGGC---------------CUCGU-GGUCCCG-GUCGAGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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