Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30700 | 5' | -47.6 | NC_006552.1 | + | 35236 | 0.66 | 0.99538 |
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Target: 5'- ----gUCCaGGCCGACUACGUaCAUgGu -3' miRNA: 3'- ccuaaAGG-CUGGCUGAUGUA-GUAgCu -5' |
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| 30700 | 5' | -47.6 | NC_006552.1 | + | 2746 | 0.66 | 0.993632 |
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Target: 5'- cGGcgUUCCGgcGCCG-CUGCGccUCAcCGAg -3' miRNA: 3'- -CCuaAAGGC--UGGCuGAUGU--AGUaGCU- -5' |
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| 30700 | 5' | -47.6 | NC_006552.1 | + | 41864 | 0.67 | 0.99139 |
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Target: 5'- uGGA---CCGuuaucGCCGGCUGCuccugccgGUCGUCGAa -3' miRNA: 3'- -CCUaaaGGC-----UGGCUGAUG--------UAGUAGCU- -5' |
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| 30700 | 5' | -47.6 | NC_006552.1 | + | 20511 | 0.67 | 0.986907 |
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Target: 5'- uGGAUUUCCGgcgucauaagcACCGuuuCaGCcUCGUCGAa -3' miRNA: 3'- -CCUAAAGGC-----------UGGCu--GaUGuAGUAGCU- -5' |
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| 30700 | 5' | -47.6 | NC_006552.1 | + | 7065 | 0.68 | 0.972814 |
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Target: 5'- uGGAagaCCGcaGCCGACUGCAUCgacugGUCGu -3' miRNA: 3'- -CCUaaaGGC--UGGCUGAUGUAG-----UAGCu -5' |
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| 30700 | 5' | -47.6 | NC_006552.1 | + | 47827 | 0.69 | 0.96629 |
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Target: 5'- cGGGUUg-CG-CCGACUGCGUUugcugGUCGAu -3' miRNA: 3'- -CCUAAagGCuGGCUGAUGUAG-----UAGCU- -5' |
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| 30700 | 5' | -47.6 | NC_006552.1 | + | 19624 | 0.69 | 0.96033 |
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Target: 5'- cGGAgauuUUUCCGACCuGcCUGCAUCGgauuuuucuccgaccUCGGa -3' miRNA: 3'- -CCU----AAAGGCUGG-CuGAUGUAGU---------------AGCU- -5' |
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| 30700 | 5' | -47.6 | NC_006552.1 | + | 5933 | 1.12 | 0.005805 |
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Target: 5'- gGGAUUUCCGACCGACUACAUCAUCGAc -3' miRNA: 3'- -CCUAAAGGCUGGCUGAUGUAGUAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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