Results 1 - 20 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30702 | 3' | -52.3 | NC_006552.1 | + | 9522 | 0.66 | 0.910379 |
Target: 5'- -aCUUCAUCGGcugccaggaAgGGGUCGAUcgCCGGg -3' miRNA: 3'- cgGAAGUAGCU---------UgUCCGGUUGa-GGCC- -5' |
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30702 | 3' | -52.3 | NC_006552.1 | + | 20449 | 0.66 | 0.903775 |
Target: 5'- gGCC-UCGuUCGGcuCGGGCCGACcgUCGGc -3' miRNA: 3'- -CGGaAGU-AGCUu-GUCCGGUUGa-GGCC- -5' |
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30702 | 3' | -52.3 | NC_006552.1 | + | 4828 | 0.66 | 0.9031 |
Target: 5'- gGCCa--GUCGcuagaccacaAGCGGGCCAACUCgcagaaaCGGg -3' miRNA: 3'- -CGGaagUAGC----------UUGUCCGGUUGAG-------GCC- -5' |
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30702 | 3' | -52.3 | NC_006552.1 | + | 1382 | 0.66 | 0.896902 |
Target: 5'- -gCUUCggCGccCAGGCCAGCcagccgCCGGc -3' miRNA: 3'- cgGAAGuaGCuuGUCCGGUUGa-----GGCC- -5' |
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30702 | 3' | -52.3 | NC_006552.1 | + | 35246 | 0.66 | 0.896902 |
Target: 5'- aGUUcUCAUCGucCAGGCCGACUaCGu -3' miRNA: 3'- -CGGaAGUAGCuuGUCCGGUUGAgGCc -5' |
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30702 | 3' | -52.3 | NC_006552.1 | + | 1164 | 0.66 | 0.889761 |
Target: 5'- cGCCUgCAUCGGuagagcagGCAGGCgGGgaUCGGu -3' miRNA: 3'- -CGGAaGUAGCU--------UGUCCGgUUgaGGCC- -5' |
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30702 | 3' | -52.3 | NC_006552.1 | + | 55300 | 0.66 | 0.889033 |
Target: 5'- cGCCcUCcUCGAACAccuggauagcaucGGUCAGCgccuugCCGGu -3' miRNA: 3'- -CGGaAGuAGCUUGU-------------CCGGUUGa-----GGCC- -5' |
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30702 | 3' | -52.3 | NC_006552.1 | + | 58573 | 0.67 | 0.882359 |
Target: 5'- gGCCcgauaCAUCGAGCGGaGCCccaggcAGC-CCGGa -3' miRNA: 3'- -CGGaa---GUAGCUUGUC-CGG------UUGaGGCC- -5' |
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30702 | 3' | -52.3 | NC_006552.1 | + | 6502 | 0.67 | 0.874699 |
Target: 5'- cGCCguugCAUCGGcACAccuGGUCAAgUUCCGGu -3' miRNA: 3'- -CGGaa--GUAGCU-UGU---CCGGUU-GAGGCC- -5' |
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30702 | 3' | -52.3 | NC_006552.1 | + | 31641 | 0.67 | 0.866789 |
Target: 5'- gGCCgaCAgc-AGCAGGCCAGCgCCGa -3' miRNA: 3'- -CGGaaGUagcUUGUCCGGUUGaGGCc -5' |
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30702 | 3' | -52.3 | NC_006552.1 | + | 57251 | 0.67 | 0.853628 |
Target: 5'- cGCgCUUCGucUCGAACAGGCUGAaguauucacgcugacCUCCu- -3' miRNA: 3'- -CG-GAAGU--AGCUUGUCCGGUU---------------GAGGcc -5' |
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30702 | 3' | -52.3 | NC_006552.1 | + | 26971 | 0.68 | 0.841624 |
Target: 5'- aGCagaUCAUUGGACAGGUaGGCggcgCCGGu -3' miRNA: 3'- -CGga-AGUAGCUUGUCCGgUUGa---GGCC- -5' |
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30702 | 3' | -52.3 | NC_006552.1 | + | 57902 | 0.68 | 0.841624 |
Target: 5'- cGCgCUUUAaUGccCGGGCCAGgUCCGGu -3' miRNA: 3'- -CG-GAAGUaGCuuGUCCGGUUgAGGCC- -5' |
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30702 | 3' | -52.3 | NC_006552.1 | + | 38775 | 0.69 | 0.785617 |
Target: 5'- -aCUUCcgCGAaggcgaucagcgACGGcGCCGACUCCuGGg -3' miRNA: 3'- cgGAAGuaGCU------------UGUC-CGGUUGAGG-CC- -5' |
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30702 | 3' | -52.3 | NC_006552.1 | + | 11002 | 0.7 | 0.734473 |
Target: 5'- cGCCUcacccaaccCAUCGAcACAacGGCCAgcGCUCUGGa -3' miRNA: 3'- -CGGAa--------GUAGCU-UGU--CCGGU--UGAGGCC- -5' |
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30702 | 3' | -52.3 | NC_006552.1 | + | 44136 | 0.7 | 0.734473 |
Target: 5'- gGCCagcaggUCGUCGAuauuCAGGCCcccaggGGCUuCCGGc -3' miRNA: 3'- -CGGa-----AGUAGCUu---GUCCGG------UUGA-GGCC- -5' |
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30702 | 3' | -52.3 | NC_006552.1 | + | 33772 | 0.7 | 0.713216 |
Target: 5'- gGUCUUCGcggugagagUGAcCGGGCCAGCgaUCCGGg -3' miRNA: 3'- -CGGAAGUa--------GCUuGUCCGGUUG--AGGCC- -5' |
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30702 | 3' | -52.3 | NC_006552.1 | + | 64157 | 0.7 | 0.702462 |
Target: 5'- uGCCgggCAUCacGCAGGCuCAACUCaGGa -3' miRNA: 3'- -CGGaa-GUAGcuUGUCCG-GUUGAGgCC- -5' |
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30702 | 3' | -52.3 | NC_006552.1 | + | 25832 | 0.71 | 0.679668 |
Target: 5'- gGCCggCAgcgguacggaaacUUGAACGGGuCCGACUCgCGGc -3' miRNA: 3'- -CGGaaGU-------------AGCUUGUCC-GGUUGAG-GCC- -5' |
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30702 | 3' | -52.3 | NC_006552.1 | + | 51573 | 0.71 | 0.669833 |
Target: 5'- cGCCUU-GUCGGcACGGGCCuGCUCggCGGc -3' miRNA: 3'- -CGGAAgUAGCU-UGUCCGGuUGAG--GCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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