Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30706 | 5' | -63 | NC_006552.1 | + | 30371 | 0.69 | 0.2349 |
Target: 5'- -cGGCCGAGGCgGCGCagGuCGuaGGGg -3' miRNA: 3'- gaCCGGCUUCGgUGCGa-C-GCggCCCa -5' |
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30706 | 5' | -63 | NC_006552.1 | + | 53241 | 0.68 | 0.252842 |
Target: 5'- -aGGUCGGAGCgCaugGCGgUGUGCUGGGg -3' miRNA: 3'- gaCCGGCUUCG-G---UGCgACGCGGCCCa -5' |
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30706 | 5' | -63 | NC_006552.1 | + | 39642 | 0.67 | 0.29837 |
Target: 5'- -cGGCCGAaguacucgccaccGGCCaguACGCUGaGCCGcGGg -3' miRNA: 3'- gaCCGGCU-------------UCGG---UGCGACgCGGC-CCa -5' |
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30706 | 5' | -63 | NC_006552.1 | + | 49641 | 0.67 | 0.299074 |
Target: 5'- uUGGCguCGggGCUugcUGCUGCGCCGccuGGUc -3' miRNA: 3'- gACCG--GCuuCGGu--GCGACGCGGC---CCA- -5' |
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30706 | 5' | -63 | NC_006552.1 | + | 46350 | 0.66 | 0.384403 |
Target: 5'- cCUGGCUGGAGCUuuGCagcaucagcagUGCGCUGacGGUg -3' miRNA: 3'- -GACCGGCUUCGGugCG-----------ACGCGGC--CCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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