Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 30706 | 5' | -63 | NC_006552.1 | + | 46350 | 0.66 | 0.384403 |
|
Target: 5'- cCUGGCUGGAGCUuuGCagcaucagcagUGCGCUGacGGUg -3' miRNA: 3'- -GACCGGCUUCGGugCG-----------ACGCGGC--CCA- -5' |
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| 30706 | 5' | -63 | NC_006552.1 | + | 49641 | 0.67 | 0.299074 |
|
Target: 5'- uUGGCguCGggGCUugcUGCUGCGCCGccuGGUc -3' miRNA: 3'- gACCG--GCuuCGGu--GCGACGCGGC---CCA- -5' |
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| 30706 | 5' | -63 | NC_006552.1 | + | 53241 | 0.68 | 0.252842 |
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Target: 5'- -aGGUCGGAGCgCaugGCGgUGUGCUGGGg -3' miRNA: 3'- gaCCGGCUUCG-G---UGCgACGCGGCCCa -5' |
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| 30706 | 5' | -63 | NC_006552.1 | + | 57088 | 0.67 | 0.320806 |
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Target: 5'- gUGGCgCGuuccGGCCAUGCaucagccaucUGCGCCaGGUg -3' miRNA: 3'- gACCG-GCu---UCGGUGCG----------ACGCGGcCCA- -5' |
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| 30706 | 5' | -63 | NC_006552.1 | + | 61618 | 0.73 | 0.111144 |
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Target: 5'- gCUGGCCG-AGCCGC-CgGCGCCGGc- -3' miRNA: 3'- -GACCGGCuUCGGUGcGaCGCGGCCca -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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