Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 30706 | 5' | -63 | NC_006552.1 | + | 5148 | 0.66 | 0.359601 |
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Target: 5'- gUGGuuGggGCUGCGCUcgaggccugcgGCGgUCGGGc -3' miRNA: 3'- gACCggCuuCGGUGCGA-----------CGC-GGCCCa -5' |
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| 30706 | 5' | -63 | NC_006552.1 | + | 3708 | 0.67 | 0.328307 |
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Target: 5'- cCUGGCCGGucGaCUACGUgcGCGCCcGGGc -3' miRNA: 3'- -GACCGGCUu-C-GGUGCGa-CGCGG-CCCa -5' |
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| 30706 | 5' | -63 | NC_006552.1 | + | 3507 | 0.67 | 0.31052 |
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Target: 5'- cCUGGCCGcucGaCCACGCcggcauccucaccGCGCgCGGGUa -3' miRNA: 3'- -GACCGGCuu-C-GGUGCGa------------CGCG-GCCCA- -5' |
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| 30706 | 5' | -63 | NC_006552.1 | + | 2227 | 0.69 | 0.218039 |
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Target: 5'- aCUGGCCGgcGCCguucuucugccgGCGgacCUGCGCCGccaGGUg -3' miRNA: 3'- -GACCGGCuuCGG------------UGC---GACGCGGC---CCA- -5' |
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| 30706 | 5' | -63 | NC_006552.1 | + | 557 | 0.66 | 0.343698 |
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Target: 5'- -cGGCCGAcGCUguuCGCcGCGCCGccGGUc -3' miRNA: 3'- gaCCGGCUuCGGu--GCGaCGCGGC--CCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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