Results 21 - 23 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30711 | 5' | -57.9 | NC_006552.1 | + | 59582 | 0.67 | 0.531992 |
Target: 5'- cGGucagcGCGGCAACCAGCACgCGGuuacGGCUg- -3' miRNA: 3'- aCC-----UGCUGUUGGUCGUG-GCC----CUGGac -5' |
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30711 | 5' | -57.9 | NC_006552.1 | + | 61617 | 0.68 | 0.48135 |
Target: 5'- cUGGcCGAgCcGCCGGCGCCGgcaGGACUUGc -3' miRNA: 3'- -ACCuGCU-GuUGGUCGUGGC---CCUGGAC- -5' |
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30711 | 5' | -57.9 | NC_006552.1 | + | 63246 | 0.67 | 0.584426 |
Target: 5'- gUGGACGACGAg-GGCAUCuaccacGGACCUGc -3' miRNA: 3'- -ACCUGCUGUUggUCGUGGc-----CCUGGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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