Results 21 - 23 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30711 | 5' | -57.9 | NC_006552.1 | + | 3671 | 0.69 | 0.423866 |
Target: 5'- cGGcguCGAUcACCAGgACCuGGACCUGu -3' miRNA: 3'- aCCu--GCUGuUGGUCgUGGcCCUGGAC- -5' |
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30711 | 5' | -57.9 | NC_006552.1 | + | 3535 | 0.72 | 0.29268 |
Target: 5'- cGGACGucCAACCuGGcCGCCGcacGGACCUGg -3' miRNA: 3'- aCCUGCu-GUUGG-UC-GUGGC---CCUGGAC- -5' |
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30711 | 5' | -57.9 | NC_006552.1 | + | 2234 | 0.66 | 0.59399 |
Target: 5'- cGcACGAacuGGCCGGCGCCGuucuucugccggcGGACCUGc -3' miRNA: 3'- aCcUGCUg--UUGGUCGUGGC-------------CCUGGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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