miRNA: 3'- uCC-GUCGgaUGCG---UGGGCGUUCACCG- -5'
miRNA: 3'- -UCcGUCGGa-UGCGUGGGCGUUCaCCG- -5'
miRNA: 3'- -UCCGUCGGAUGCGUgGGCGUUCACCG- -5'
Results 41 - 43 of 43 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30718 | 3' | -58.8 | NC_006552.1 | + | 39776 | 0.79 | 0.075169 |
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Target: 5'- cGGuCAGCg-GCGUgucGCCCGCAGGUGGCa -3' miRNA: 3'- uCC-GUCGgaUGCG---UGGGCGUUCACCG- -5' |
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| 30718 | 3' | -58.8 | NC_006552.1 | + | 5799 | 0.8 | 0.073089 |
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Target: 5'- aAGcCAGCCcaACGCGCCCGCAAG-GGCg -3' miRNA: 3'- -UCcGUCGGa-UGCGUGGGCGUUCaCCG- -5' |
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| 30718 | 3' | -58.8 | NC_006552.1 | + | 53876 | 1 | 0.002494 |
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Target: 5'- aAGGCAGCCUACGCA-CCGCAAGUGGCa -3' miRNA: 3'- -UCCGUCGGAUGCGUgGGCGUUCACCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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