Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30758 | 3' | -54.5 | NC_006552.1 | + | 59328 | 0.67 | 0.722978 |
Target: 5'- aGGUGcauaugugcgguaacAGCGUCAGCCCGccGCCGaugGCa -3' miRNA: 3'- cCCAC---------------UUGUAGUCGGGCcuCGGUa--UG- -5' |
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30758 | 3' | -54.5 | NC_006552.1 | + | 57769 | 0.66 | 0.760522 |
Target: 5'- uGGGU---CAUCAGCCUGGAGaaCGUGa -3' miRNA: 3'- -CCCAcuuGUAGUCGGGCCUCg-GUAUg -5' |
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30758 | 3' | -54.5 | NC_006552.1 | + | 32797 | 1.12 | 0.00082 |
Target: 5'- uGGGUGAACAUCAGCCCGGAGCCAUACa -3' miRNA: 3'- -CCCACUUGUAGUCGGGCCUCGGUAUG- -5' |
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30758 | 3' | -54.5 | NC_006552.1 | + | 31839 | 0.71 | 0.492193 |
Target: 5'- cGGGUGAugcGCcgCAGgUCGGAGUCGggGCg -3' miRNA: 3'- -CCCACU---UGuaGUCgGGCCUCGGUa-UG- -5' |
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30758 | 3' | -54.5 | NC_006552.1 | + | 31269 | 0.72 | 0.422972 |
Target: 5'- gGGGUGAuCggCAGgCCGGuAGCCGUGu -3' miRNA: 3'- -CCCACUuGuaGUCgGGCC-UCGGUAUg -5' |
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30758 | 3' | -54.5 | NC_006552.1 | + | 17908 | 0.68 | 0.642717 |
Target: 5'- uGGcGAACGcuaUCGGCCCGaGAGCUAUc- -3' miRNA: 3'- cCCaCUUGU---AGUCGGGC-CUCGGUAug -5' |
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30758 | 3' | -54.5 | NC_006552.1 | + | 11566 | 0.66 | 0.769664 |
Target: 5'- cGGUGAucuugcugccacuGCGcCGGCCCaGGGCC-UGCa -3' miRNA: 3'- cCCACU-------------UGUaGUCGGGcCUCGGuAUG- -5' |
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30758 | 3' | -54.5 | NC_006552.1 | + | 1998 | 0.68 | 0.697268 |
Target: 5'- uGGGcu-GCAUCAGCCUGGAuCCGc-- -3' miRNA: 3'- -CCCacuUGUAGUCGGGCCUcGGUaug -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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