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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 30759 | 5' | -60.3 | NC_006552.1 | + | 32501 | 1.07 | 0.000608 |
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Target: 5'- gCGCGUGGACGGCAUCACGGGAGCGCGc -3' miRNA: 3'- -GCGCACCUGCCGUAGUGCCCUCGCGC- -5' |
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| 30759 | 5' | -60.3 | NC_006552.1 | + | 1915 | 0.72 | 0.24299 |
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Target: 5'- aCGaCGUGcucgacGGCGGCGUCGuuguCGGGAGUGUGg -3' miRNA: 3'- -GC-GCAC------CUGCCGUAGU----GCCCUCGCGC- -5' |
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| 30759 | 5' | -60.3 | NC_006552.1 | + | 52959 | 0.72 | 0.24299 |
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Target: 5'- cCGCc-GGuGCGGCAUC-CGGGuGCGCGu -3' miRNA: 3'- -GCGcaCC-UGCCGUAGuGCCCuCGCGC- -5' |
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| 30759 | 5' | -60.3 | NC_006552.1 | + | 25307 | 0.69 | 0.33136 |
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Target: 5'- gGUGUcGGuCGGCGUCcaagcuggGCGGGuAGCGCu -3' miRNA: 3'- gCGCA-CCuGCCGUAG--------UGCCC-UCGCGc -5' |
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| 30759 | 5' | -60.3 | NC_006552.1 | + | 22518 | 0.68 | 0.396517 |
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Target: 5'- --aGUGGugGGCcgCcuugugauCGGcGAGCGCGg -3' miRNA: 3'- gcgCACCugCCGuaGu-------GCC-CUCGCGC- -5' |
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| 30759 | 5' | -60.3 | NC_006552.1 | + | 50097 | 0.68 | 0.42299 |
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Target: 5'- aCGCGggcGGaACGcGCAgCACGGcGGCGCGu -3' miRNA: 3'- -GCGCa--CC-UGC-CGUaGUGCCcUCGCGC- -5' |
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| 30759 | 5' | -60.3 | NC_006552.1 | + | 28225 | 0.66 | 0.53831 |
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Target: 5'- uCGCGaccucCGGCGUCACGuuguGGAuGCGCGg -3' miRNA: 3'- -GCGCaccu-GCCGUAGUGC----CCU-CGCGC- -5' |
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| 30759 | 5' | -60.3 | NC_006552.1 | + | 5661 | 0.66 | 0.53831 |
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Target: 5'- aCGCccccGGAUGGCcUCgauGCGGGcAGCGCu -3' miRNA: 3'- -GCGca--CCUGCCGuAG---UGCCC-UCGCGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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