Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 30791 | 3' | -64.8 | NC_006552.1 | + | 25400 | 0.7 | 0.155106 |
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Target: 5'- -gGUCCAGUUagcagcaggugcugCCGUCCCAGCgaacaggGCCAGGa -3' miRNA: 3'- agCGGGUCAG--------------GGCGGGGUCG-------CGGUCC- -5' |
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| 30791 | 3' | -64.8 | NC_006552.1 | + | 44068 | 0.7 | 0.164839 |
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Target: 5'- cCGCCCGGcgcgcuuccucuUCgCCGCCgcgcucuuggCCGGCGCCuGGg -3' miRNA: 3'- aGCGGGUC------------AG-GGCGG----------GGUCGCGGuCC- -5' |
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| 30791 | 3' | -64.8 | NC_006552.1 | + | 20193 | 0.69 | 0.206368 |
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Target: 5'- gCGCugCUGGUugaUCUGCCCCAGCGCCAc- -3' miRNA: 3'- aGCG--GGUCA---GGGCGGGGUCGCGGUcc -5' |
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| 30791 | 3' | -64.8 | NC_006552.1 | + | 32973 | 0.69 | 0.206368 |
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Target: 5'- gCGUCgGcGUCCUgGCuCCCAGCGuCCGGGa -3' miRNA: 3'- aGCGGgU-CAGGG-CG-GGGUCGC-GGUCC- -5' |
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| 30791 | 3' | -64.8 | NC_006552.1 | + | 16068 | 0.69 | 0.206368 |
|
Target: 5'- -aGCCgGGUCCaGCCCCaucugcucggAGCGCUuuGGGg -3' miRNA: 3'- agCGGgUCAGGgCGGGG----------UCGCGG--UCC- -5' |
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| 30791 | 3' | -64.8 | NC_006552.1 | + | 61443 | 0.66 | 0.338696 |
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Target: 5'- gCGCCCGGg-CCGUacggcaauaCgAGUGCCGGGa -3' miRNA: 3'- aGCGGGUCagGGCGg--------GgUCGCGGUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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