Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30792 | 5' | -58.1 | NC_006552.1 | + | 8429 | 0.66 | 0.591426 |
Target: 5'- cCGCGcCAGGCGCGCagcaGUCCcucGGGa- -3' miRNA: 3'- -GCGC-GUUCGCGCGaag-UAGGu--CCCgu -5' |
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30792 | 5' | -58.1 | NC_006552.1 | + | 31579 | 0.66 | 0.591426 |
Target: 5'- aGCGCuGGCGgGCauagUCAUagAGGGUAu -3' miRNA: 3'- gCGCGuUCGCgCGa---AGUAggUCCCGU- -5' |
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30792 | 5' | -58.1 | NC_006552.1 | + | 15020 | 0.66 | 0.591426 |
Target: 5'- gGCGCcAGUG-GCUUCAgcaCgCGGGGCu -3' miRNA: 3'- gCGCGuUCGCgCGAAGUa--G-GUCCCGu -5' |
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30792 | 5' | -58.1 | NC_006552.1 | + | 17957 | 0.66 | 0.584993 |
Target: 5'- gCGCGcCGAGUuaccgGCGUUguacugagccaacgCGUCCGGGGCc -3' miRNA: 3'- -GCGC-GUUCG-----CGCGAa-------------GUAGGUCCCGu -5' |
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30792 | 5' | -58.1 | NC_006552.1 | + | 31639 | 0.66 | 0.580712 |
Target: 5'- aGCGCAcuccuGCGCGCUgc-UgCGGGGUu -3' miRNA: 3'- gCGCGUu----CGCGCGAaguAgGUCCCGu -5' |
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30792 | 5' | -58.1 | NC_006552.1 | + | 20631 | 0.66 | 0.579643 |
Target: 5'- cCGCGCGGGUG-GUUUCG-CCAcugucgcguucgcGGGCAc -3' miRNA: 3'- -GCGCGUUCGCgCGAAGUaGGU-------------CCCGU- -5' |
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30792 | 5' | -58.1 | NC_006552.1 | + | 15797 | 0.67 | 0.538341 |
Target: 5'- uCGCGCucGGCGauuuucuuCGCguccCAUCCAGuGGCAc -3' miRNA: 3'- -GCGCGu-UCGC--------GCGaa--GUAGGUC-CCGU- -5' |
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30792 | 5' | -58.1 | NC_006552.1 | + | 33539 | 0.67 | 0.517546 |
Target: 5'- uGCGCAGGCGCGCagCggCCGacaGCAg -3' miRNA: 3'- gCGCGUUCGCGCGaaGuaGGUcc-CGU- -5' |
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30792 | 5' | -58.1 | NC_006552.1 | + | 15569 | 0.68 | 0.447621 |
Target: 5'- uGCuGCu-GCGcCGCcUgGUCCAGGGCGa -3' miRNA: 3'- gCG-CGuuCGC-GCGaAgUAGGUCCCGU- -5' |
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30792 | 5' | -58.1 | NC_006552.1 | + | 62491 | 0.68 | 0.437108 |
Target: 5'- gCGCGCugaAGGUGaucgccaCGCUUCGccgCCAGGGCu -3' miRNA: 3'- -GCGCG---UUCGC-------GCGAAGUa--GGUCCCGu -5' |
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30792 | 5' | -58.1 | NC_006552.1 | + | 52945 | 0.7 | 0.374578 |
Target: 5'- uGCGCcGGCGCccgccgccgguGCggCAUCCGGGuGCGc -3' miRNA: 3'- gCGCGuUCGCG-----------CGaaGUAGGUCC-CGU- -5' |
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30792 | 5' | -58.1 | NC_006552.1 | + | 3014 | 0.7 | 0.374578 |
Target: 5'- gCGCGCgGAGCagaucGUGCUcCA-CCAGGGCGa -3' miRNA: 3'- -GCGCG-UUCG-----CGCGAaGUaGGUCCCGU- -5' |
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30792 | 5' | -58.1 | NC_006552.1 | + | 43362 | 0.71 | 0.325348 |
Target: 5'- gGCacuCAGGuCGUGCUUCcuccgaccAUCCAGGGCAu -3' miRNA: 3'- gCGc--GUUC-GCGCGAAG--------UAGGUCCCGU- -5' |
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30792 | 5' | -58.1 | NC_006552.1 | + | 23155 | 0.71 | 0.318392 |
Target: 5'- uCGCGCucgaacaccggcaccAGCGcCGCcaCGUCCAGGGUAa -3' miRNA: 3'- -GCGCGu--------------UCGC-GCGaaGUAGGUCCCGU- -5' |
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30792 | 5' | -58.1 | NC_006552.1 | + | 9391 | 0.74 | 0.201236 |
Target: 5'- cCGCGCAcgucgccccgugcGGCGCGCUUCAUgCCAGccaucgcguaGGCc -3' miRNA: 3'- -GCGCGU-------------UCGCGCGAAGUA-GGUC----------CCGu -5' |
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30792 | 5' | -58.1 | NC_006552.1 | + | 42304 | 0.75 | 0.167651 |
Target: 5'- gCGUGCAgaaacuccgguAGCGCGCUUCuucgcuaCCGGGGCc -3' miRNA: 3'- -GCGCGU-----------UCGCGCGAAGua-----GGUCCCGu -5' |
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30792 | 5' | -58.1 | NC_006552.1 | + | 15152 | 1.08 | 0.000636 |
Target: 5'- cCGCGCAAGCGCGCUUCAUCCAGGGCAu -3' miRNA: 3'- -GCGCGUUCGCGCGAAGUAGGUCCCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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