Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 30805 | 3' | -51.3 | NC_006552.1 | + | 5893 | 1.12 | 0.001996 |
|
Target: 5'- gACAUAACUCCAUCCUCCAAAGGCCGGg -3' miRNA: 3'- -UGUAUUGAGGUAGGAGGUUUCCGGCC- -5' |
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| 30805 | 3' | -51.3 | NC_006552.1 | + | 17009 | 0.69 | 0.827334 |
|
Target: 5'- ------aUCCuucgCCUCCcGGGGCCGGa -3' miRNA: 3'- uguauugAGGua--GGAGGuUUCCGGCC- -5' |
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| 30805 | 3' | -51.3 | NC_006552.1 | + | 807 | 0.68 | 0.845335 |
|
Target: 5'- cGCGaAACUUCAUgUUCgCcAGGGCCGGg -3' miRNA: 3'- -UGUaUUGAGGUAgGAG-GuUUCCGGCC- -5' |
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| 30805 | 3' | -51.3 | NC_006552.1 | + | 10964 | 0.67 | 0.88615 |
|
Target: 5'- -gGUGGCacCCAggCCUCCAGccAGGCaCGGa -3' miRNA: 3'- ugUAUUGa-GGUa-GGAGGUU--UCCG-GCC- -5' |
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| 30805 | 3' | -51.3 | NC_006552.1 | + | 22134 | 0.67 | 0.907481 |
|
Target: 5'- cCGUGGCaCCGUCCaCCGucAGGCCGc -3' miRNA: 3'- uGUAUUGaGGUAGGaGGUu-UCCGGCc -5' |
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| 30805 | 3' | -51.3 | NC_006552.1 | + | 39160 | 0.66 | 0.914034 |
|
Target: 5'- gACGUGccGCcuUCCAUCaUUCCGAgaucGGGCUGGc -3' miRNA: 3'- -UGUAU--UG--AGGUAG-GAGGUU----UCCGGCC- -5' |
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| 30805 | 3' | -51.3 | NC_006552.1 | + | 5579 | 0.66 | 0.920305 |
|
Target: 5'- ---cGACUUCAUCCaagcgCCGAAG-CCGGc -3' miRNA: 3'- uguaUUGAGGUAGGa----GGUUUCcGGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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