Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30809 | 5' | -55.3 | NC_006552.1 | + | 21648 | 0.66 | 0.742047 |
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Target: 5'- cUGCCGGcCaCCGgcugGCUGUCaccguCCAGCg -3' miRNA: 3'- cAUGGCCuGcGGCaa--CGAUAG-----GGUCG- -5' |
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| 30809 | 5' | -55.3 | NC_006552.1 | + | 45780 | 0.66 | 0.752328 |
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Target: 5'- -aAgCGGuACGCCGUguccgGCUuGUCCguCAGCa -3' miRNA: 3'- caUgGCC-UGCGGCAa----CGA-UAGG--GUCG- -5' |
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| 30809 | 5' | -55.3 | NC_006552.1 | + | 34750 | 0.66 | 0.762489 |
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Target: 5'- uUACCacGGGCGCCGgccagGUgaa-CCAGCa -3' miRNA: 3'- cAUGG--CCUGCGGCaa---CGauagGGUCG- -5' |
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| 30809 | 5' | -55.3 | NC_006552.1 | + | 33218 | 0.66 | 0.771524 |
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Target: 5'- -gGCCaGGAUGCCGc-GCUGcaugucuUCCgGGCa -3' miRNA: 3'- caUGG-CCUGCGGCaaCGAU-------AGGgUCG- -5' |
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| 30809 | 5' | -55.3 | NC_006552.1 | + | 41157 | 0.66 | 0.773516 |
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Target: 5'- -cGCUGGuCGCCGUUGaacagcgcauggUCCAGCc -3' miRNA: 3'- caUGGCCuGCGGCAACgaua--------GGGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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