miRNA: 3'- cUCGCGAUG-----UUGCaa---CGCGGUAGCUG- -5'
miRNA: 3'- -CUCGCGAUGu-----------UGCaACGCGgUAGcUG- -5'
miRNA: 3'- cUCGCGAUGUUGCaacGC-GGuAGCUG- -5'
miRNA: 3'- cucGCGAUGuuGCAAc-GC-GGUAGCUG- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30812 | 3' | -53.2 | NC_006552.1 | + | 52129 | 0.66 | 0.834788 |
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Target: 5'- cAGCGCUACccaguGGCGcgcccGCGUCGUUGAUa -3' miRNA: 3'- cUCGCGAUG-----UUGCaa---CGCGGUAGCUG- -5' |
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| 30812 | 3' | -53.2 | NC_006552.1 | + | 4923 | 0.66 | 0.841925 |
|
Target: 5'- uGAGCGCUGCcuguuucaugucGCGcUGCGCgGUCaGCu -3' miRNA: 3'- -CUCGCGAUGu-----------UGCaACGCGgUAGcUG- -5' |
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| 30812 | 3' | -53.2 | NC_006552.1 | + | 24678 | 0.66 | 0.852358 |
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Target: 5'- aAGCGUUGCAACGccaCGuCCuUCGGCg -3' miRNA: 3'- cUCGCGAUGUUGCaacGC-GGuAGCUG- -5' |
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| 30812 | 3' | -53.2 | NC_006552.1 | + | 16883 | 0.66 | 0.852358 |
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Target: 5'- --cCGCUACggUGUUcaCGaCCAUCGACg -3' miRNA: 3'- cucGCGAUGuuGCAAc-GC-GGUAGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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