Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 30823 | 3' | -57.2 | NC_006552.1 | + | 45425 | 0.66 | 0.681799 |
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Target: 5'- --cGggGCCGGcGCAGC-CuccCCGGCa -3' miRNA: 3'- cuuCuuCGGCC-UGUCGuGccaGGCCG- -5' |
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| 30823 | 3' | -57.2 | NC_006552.1 | + | 47688 | 0.66 | 0.671146 |
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Target: 5'- cGAGAaagcauucgcccAGCCGGAggUGGCGCGuaacgCCGGCc -3' miRNA: 3'- cUUCU------------UCGGCCU--GUCGUGCca---GGCCG- -5' |
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| 30823 | 3' | -57.2 | NC_006552.1 | + | 53591 | 0.67 | 0.596131 |
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Target: 5'- --uGgcGCCGGcCAGUuCGGUgccgCCGGCu -3' miRNA: 3'- cuuCuuCGGCCuGUCGuGCCA----GGCCG- -5' |
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| 30823 | 3' | -57.2 | NC_006552.1 | + | 55153 | 0.66 | 0.671146 |
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Target: 5'- cAAGAAGCCcGACGGCACcccGaCCGuGCu -3' miRNA: 3'- cUUCUUCGGcCUGUCGUGc--CaGGC-CG- -5' |
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| 30823 | 3' | -57.2 | NC_006552.1 | + | 59921 | 0.72 | 0.337911 |
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Target: 5'- cAAGGcgcGCCGGACcGUGCuGUCCGGCu -3' miRNA: 3'- cUUCUu--CGGCCUGuCGUGcCAGGCCG- -5' |
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| 30823 | 3' | -57.2 | NC_006552.1 | + | 61270 | 0.69 | 0.48197 |
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Target: 5'- aGGGAAGCUGGGCcGCAaacccaugGGUCUGGg -3' miRNA: 3'- cUUCUUCGGCCUGuCGUg-------CCAGGCCg -5' |
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| 30823 | 3' | -57.2 | NC_006552.1 | + | 61369 | 0.73 | 0.271751 |
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Target: 5'- -cGGGAGCCGGGCcauguuuuGGCGCG--CCGGCg -3' miRNA: 3'- cuUCUUCGGCCUG--------UCGUGCcaGGCCG- -5' |
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| 30823 | 3' | -57.2 | NC_006552.1 | + | 61648 | 0.68 | 0.547414 |
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Target: 5'- cGAGGccgccGGCCGGACGuccaaccuggccgccGCACGGaCCuGGCc -3' miRNA: 3'- -CUUCu----UCGGCCUGU---------------CGUGCCaGG-CCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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