Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30856 | 3' | -61.1 | NC_006552.1 | + | 63083 | 0.66 | 0.454039 |
Target: 5'- gGCCAAcGcCGUCgCCGAGaacuGCCGuGCGAu -3' miRNA: 3'- -CGGUUcC-GCAGaGGCUCg---CGGC-CGCU- -5' |
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30856 | 3' | -61.1 | NC_006552.1 | + | 35371 | 0.66 | 0.472962 |
Target: 5'- cGCCcGGGaua-UCCGAGagacugGCCGGCGGu -3' miRNA: 3'- -CGGuUCCgcagAGGCUCg-----CGGCCGCU- -5' |
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30856 | 3' | -61.1 | NC_006552.1 | + | 55284 | 0.66 | 0.479679 |
Target: 5'- uCCAGGGCGagUCCGgggccuacccguucAGCGgcuggcUCGGCGAg -3' miRNA: 3'- cGGUUCCGCagAGGC--------------UCGC------GGCCGCU- -5' |
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30856 | 3' | -61.1 | NC_006552.1 | + | 13162 | 0.66 | 0.482572 |
Target: 5'- aCCAGGGCGUCg-CGGGCGUUcuGCGu -3' miRNA: 3'- cGGUUCCGCAGagGCUCGCGGc-CGCu -5' |
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30856 | 3' | -61.1 | NC_006552.1 | + | 7194 | 0.66 | 0.492274 |
Target: 5'- cCCAAGcGCGUC-CCGuccccGGCGCgCaGCGAg -3' miRNA: 3'- cGGUUC-CGCAGaGGC-----UCGCG-GcCGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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