Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30856 | 3' | -61.1 | NC_006552.1 | + | 64798 | 0.7 | 0.277139 |
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Target: 5'- uGCCGGaaacGGCGUUUCCGGccGgGCCGGUa- -3' miRNA: 3'- -CGGUU----CCGCAGAGGCU--CgCGGCCGcu -5' |
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| 30856 | 3' | -61.1 | NC_006552.1 | + | 22187 | 0.7 | 0.275152 |
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Target: 5'- aCCAGGGCGUCaucgaCGugcaggacacgaagGGUGCCGGUGAc -3' miRNA: 3'- cGGUUCCGCAGag---GC--------------UCGCGGCCGCU- -5' |
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| 30856 | 3' | -61.1 | NC_006552.1 | + | 20936 | 0.72 | 0.206275 |
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Target: 5'- aCCGuGGUGUCgaugUCCGGGCGuCCGGCa- -3' miRNA: 3'- cGGUuCCGCAG----AGGCUCGC-GGCCGcu -5' |
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| 30856 | 3' | -61.1 | NC_006552.1 | + | 3570 | 0.72 | 0.196111 |
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Target: 5'- cGCC-AGGUG-CUggCCGAGcCGCCGGCGc -3' miRNA: 3'- -CGGuUCCGCaGA--GGCUC-GCGGCCGCu -5' |
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| 30856 | 3' | -61.1 | NC_006552.1 | + | 4732 | 0.72 | 0.195615 |
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Target: 5'- gGCCGAggcGGCGUCUCCuGcaccagcuucggcGGUGCCGGCu- -3' miRNA: 3'- -CGGUU---CCGCAGAGG-C-------------UCGCGGCCGcu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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