miRNA: 3'- ggaCUGCGa--CCUGUGGG-GCCGCGGcG- -5'
miRNA: 3'- -GGaCUGCGac-----CUGUggGGCCGCGGCG- -5'
miRNA: 3'- gGAC--UGC----GACCUGUGG-GGCCGCGGCg -5'
Results 41 - 43 of 43 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30882 | 3' | -62 | NC_006552.1 | + | 14770 | 0.71 | 0.206485 |
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Target: 5'- --cGGCGCgagGGAUGCCCaCGGUGCC-Cg -3' miRNA: 3'- ggaCUGCGa--CCUGUGGG-GCCGCGGcG- -5' |
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| 30882 | 3' | -62 | NC_006552.1 | + | 62435 | 0.72 | 0.196439 |
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Target: 5'- cCCcGACGUgcccgccGGCGuuCCGGCGCCGCu -3' miRNA: 3'- -GGaCUGCGac-----CUGUggGGCCGCGGCG- -5' |
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| 30882 | 3' | -62 | NC_006552.1 | + | 65072 | 0.78 | 0.07681 |
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Target: 5'- aCUGcaACGgaacCUGGACGCCgCCGGCGCCGg -3' miRNA: 3'- gGAC--UGC----GACCUGUGG-GGCCGCGGCg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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