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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 30923 | 5' | -54.5 | NC_006552.1 | + | 10819 | 0.76 | 0.275113 |
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Target: 5'- aGUACUgcacaggcgguUGUUCGCCGGGCuGCGGcgcUGCCGa -3' miRNA: 3'- -CAUGA-----------GCAAGCGGCCUG-CGUU---ACGGC- -5' |
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| 30923 | 5' | -54.5 | NC_006552.1 | + | 32688 | 0.75 | 0.303883 |
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Target: 5'- cUGCUUGgugucgUCGCCGGccgGCGCGggGCCGg -3' miRNA: 3'- cAUGAGCa-----AGCGGCC---UGCGUuaCGGC- -5' |
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| 30923 | 5' | -54.5 | NC_006552.1 | + | 29548 | 0.67 | 0.726283 |
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Target: 5'- aGUACg-GaUCGCCGGugGCGGUaCCa -3' miRNA: 3'- -CAUGagCaAGCGGCCugCGUUAcGGc -5' |
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| 30923 | 5' | -54.5 | NC_006552.1 | + | 12600 | 0.67 | 0.736747 |
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Target: 5'- uGUGCuUCGUcucgaugaugUCGCCGGcCGUAGucUGCCc -3' miRNA: 3'- -CAUG-AGCA----------AGCGGCCuGCGUU--ACGGc -5' |
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| 30923 | 5' | -54.5 | NC_006552.1 | + | 49860 | 0.67 | 0.757352 |
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Target: 5'- -gGCUCGacgccgCGUCGGACGCcc-GCUGg -3' miRNA: 3'- caUGAGCaa----GCGGCCUGCGuuaCGGC- -5' |
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| 30923 | 5' | -54.5 | NC_006552.1 | + | 36410 | 0.67 | 0.766466 |
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Target: 5'- -cGCUCGgagaCGCCuuggcuuGGGCGgCAGUGUCGg -3' miRNA: 3'- caUGAGCaa--GCGG-------CCUGC-GUUACGGC- -5' |
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| 30923 | 5' | -54.5 | NC_006552.1 | + | 37013 | 0.67 | 0.767472 |
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Target: 5'- cUGCUCuucaucUUGaCCGGACGCGcUGCCa -3' miRNA: 3'- cAUGAGca----AGC-GGCCUGCGUuACGGc -5' |
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| 30923 | 5' | -54.5 | NC_006552.1 | + | 51397 | 0.66 | 0.815806 |
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Target: 5'- aGUACgCGacgcUGCCGGACGcCAAUaccGCCGa -3' miRNA: 3'- -CAUGaGCaa--GCGGCCUGC-GUUA---CGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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