Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 30932 | 5' | -56.1 | NC_006552.1 | + | 57960 | 0.75 | 0.22141 |
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Target: 5'- cACCUGCUGGAGgGGauggGCuACCAGGUg -3' miRNA: 3'- cUGGACGACUUCaCCg---CGuUGGUCCG- -5' |
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| 30932 | 5' | -56.1 | NC_006552.1 | + | 58390 | 0.67 | 0.593804 |
|
Target: 5'- aGACCgGCUacGGUGaGCGCGAa-GGGCa -3' miRNA: 3'- -CUGGaCGAcuUCAC-CGCGUUggUCCG- -5' |
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| 30932 | 5' | -56.1 | NC_006552.1 | + | 58599 | 0.69 | 0.508304 |
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Target: 5'- aGCCcGUUGAuggugggcgucGGUGGcCGCG-CCGGGCa -3' miRNA: 3'- cUGGaCGACU-----------UCACC-GCGUuGGUCCG- -5' |
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| 30932 | 5' | -56.1 | NC_006552.1 | + | 61769 | 0.74 | 0.263284 |
|
Target: 5'- aGGCCUGUUGcGGUGGCGgccucccucaaccaCAAgCAGGCc -3' miRNA: 3'- -CUGGACGACuUCACCGC--------------GUUgGUCCG- -5' |
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| 30932 | 5' | -56.1 | NC_006552.1 | + | 62996 | 0.67 | 0.648523 |
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Target: 5'- gGACCUGCgccgccagguguUGGAGaaaaUGGCcgcccagcGCAACCuGGCc -3' miRNA: 3'- -CUGGACG------------ACUUC----ACCG--------CGUUGGuCCG- -5' |
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| 30932 | 5' | -56.1 | NC_006552.1 | + | 64795 | 0.66 | 0.702856 |
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Target: 5'- -uCCUGCcgGAAacGGCGUuuccGGCCGGGCc -3' miRNA: 3'- cuGGACGa-CUUcaCCGCG----UUGGUCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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