Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30932 | 5' | -56.1 | NC_006552.1 | + | 44570 | 0.66 | 0.659461 |
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Target: 5'- cGCCUGCUGcc-UGGCacgaGUGAgCAGGCg -3' miRNA: 3'- cUGGACGACuucACCG----CGUUgGUCCG- -5' |
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| 30932 | 5' | -56.1 | NC_006552.1 | + | 14435 | 0.66 | 0.670374 |
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Target: 5'- --gCUGCUGGcaucGGUGGUGgAACUGGGa -3' miRNA: 3'- cugGACGACU----UCACCGCgUUGGUCCg -5' |
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| 30932 | 5' | -56.1 | NC_006552.1 | + | 33522 | 0.66 | 0.692082 |
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Target: 5'- cGCCuUGCUGGAacUGGUGCG--CAGGCg -3' miRNA: 3'- cUGG-ACGACUUc-ACCGCGUugGUCCG- -5' |
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| 30932 | 5' | -56.1 | NC_006552.1 | + | 28729 | 0.66 | 0.702856 |
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Target: 5'- cGAUCUGCggGAAGccGGCcgGCGGCgaAGGCa -3' miRNA: 3'- -CUGGACGa-CUUCa-CCG--CGUUGg-UCCG- -5' |
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| 30932 | 5' | -56.1 | NC_006552.1 | + | 49393 | 0.66 | 0.702856 |
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Target: 5'- cGACaaGCUGAuGacGCGCAGUCAGGCc -3' miRNA: 3'- -CUGgaCGACUuCacCGCGUUGGUCCG- -5' |
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| 30932 | 5' | -56.1 | NC_006552.1 | + | 64795 | 0.66 | 0.702856 |
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Target: 5'- -uCCUGCcgGAAacGGCGUuuccGGCCGGGCc -3' miRNA: 3'- cuGGACGa-CUUcaCCGCG----UUGGUCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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