Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30932 | 5' | -56.1 | NC_006552.1 | + | 22006 | 0.7 | 0.456562 |
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Target: 5'- cGCCUgaucGCUGAcGGUGGCGgcguccuCGACCAGGa -3' miRNA: 3'- cUGGA----CGACU-UCACCGC-------GUUGGUCCg -5' |
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| 30932 | 5' | -56.1 | NC_006552.1 | + | 16034 | 0.7 | 0.429385 |
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Target: 5'- cGGCCUGCgcagcagcaccagaAAGUGGCccaGUAGCCGGGUc -3' miRNA: 3'- -CUGGACGac------------UUCACCG---CGUUGGUCCG- -5' |
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| 30932 | 5' | -56.1 | NC_006552.1 | + | 50269 | 0.71 | 0.391367 |
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Target: 5'- gGGCCUccGCaUGAAGccaaaugcgacUGGCGCccGCCGGGCg -3' miRNA: 3'- -CUGGA--CG-ACUUC-----------ACCGCGu-UGGUCCG- -5' |
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| 30932 | 5' | -56.1 | NC_006552.1 | + | 53612 | 0.73 | 0.300793 |
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Target: 5'- cGCCgGCUGAGGUGGagGCcugAGCCAuGGCa -3' miRNA: 3'- cUGGaCGACUUCACCg-CG---UUGGU-CCG- -5' |
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| 30932 | 5' | -56.1 | NC_006552.1 | + | 61769 | 0.74 | 0.263284 |
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Target: 5'- aGGCCUGUUGcGGUGGCGgccucccucaaccaCAAgCAGGCc -3' miRNA: 3'- -CUGGACGACuUCACCGC--------------GUUgGUCCG- -5' |
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| 30932 | 5' | -56.1 | NC_006552.1 | + | 57960 | 0.75 | 0.22141 |
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Target: 5'- cACCUGCUGGAGgGGauggGCuACCAGGUg -3' miRNA: 3'- cUGGACGACUUCaCCg---CGuUGGUCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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