Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30937 | 3' | -60.7 | NC_006552.1 | + | 32076 | 0.66 | 0.463042 |
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Target: 5'- uGCC-AGCCCGGucGGGGGuuggcaCCAGGCGc -3' miRNA: 3'- uCGGcUCGGGUUu-CUCCC------GGUCCGUc -5' |
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| 30937 | 3' | -60.7 | NC_006552.1 | + | 54352 | 0.66 | 0.443921 |
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Target: 5'- uGGCUGAucugcuGCCgCAAGGAGGagaagauccuGCCuGGCAGc -3' miRNA: 3'- -UCGGCU------CGG-GUUUCUCC----------CGGuCCGUC- -5' |
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| 30937 | 3' | -60.7 | NC_006552.1 | + | 26447 | 0.66 | 0.434528 |
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Target: 5'- cGCCGAcUCCu--GGGGGCCGcGCAGc -3' miRNA: 3'- uCGGCUcGGGuuuCUCCCGGUcCGUC- -5' |
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| 30937 | 3' | -60.7 | NC_006552.1 | + | 13320 | 0.67 | 0.389362 |
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Target: 5'- uGCCG-GUUCGGAcuGGGGCCGGGCu- -3' miRNA: 3'- uCGGCuCGGGUUUc-UCCCGGUCCGuc -5' |
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| 30937 | 3' | -60.7 | NC_006552.1 | + | 45406 | 0.67 | 0.389362 |
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Target: 5'- cGCCGAcgaauuccccGCCC----GGGGCCGGcGCAGc -3' miRNA: 3'- uCGGCU----------CGGGuuucUCCCGGUC-CGUC- -5' |
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| 30937 | 3' | -60.7 | NC_006552.1 | + | 59377 | 0.67 | 0.38762 |
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Target: 5'- cGCgCGAGCCaacgauccauGGAGGGCgcggcagcguCAGGCGGu -3' miRNA: 3'- uCG-GCUCGGguu-------UCUCCCG----------GUCCGUC- -5' |
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| 30937 | 3' | -60.7 | NC_006552.1 | + | 30644 | 0.67 | 0.372176 |
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Target: 5'- cGuuGAGCCUugGAAGucGGCCacccAGGCGGu -3' miRNA: 3'- uCggCUCGGG--UUUCucCCGG----UCCGUC- -5' |
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| 30937 | 3' | -60.7 | NC_006552.1 | + | 6618 | 0.7 | 0.241495 |
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Target: 5'- cAGCCG-GCCCGAuacaucgagcGGAGccCCAGGCAGc -3' miRNA: 3'- -UCGGCuCGGGUU----------UCUCccGGUCCGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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