Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30940 | 3' | -54.4 | NC_006552.1 | + | 58486 | 0.7 | 0.586484 |
Target: 5'- uGUCGagCGCugugcuuCUCCCGGCAACacaucaaGGCGCa -3' miRNA: 3'- -CAGCagGUGu------GAGGGCUGUUG-------CUGCG- -5' |
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30940 | 3' | -54.4 | NC_006552.1 | + | 29296 | 0.7 | 0.587557 |
Target: 5'- ---aUUCGCGCcCCaCGGCGGCGGCGCa -3' miRNA: 3'- cagcAGGUGUGaGG-GCUGUUGCUGCG- -5' |
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30940 | 3' | -54.4 | NC_006552.1 | + | 12714 | 0.7 | 0.609088 |
Target: 5'- cGUCGUagcgaauaCGCugUUCCGGCGucaGGCGCa -3' miRNA: 3'- -CAGCAg-------GUGugAGGGCUGUug-CUGCG- -5' |
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30940 | 3' | -54.4 | NC_006552.1 | + | 44087 | 0.7 | 0.619886 |
Target: 5'- uUCGccgCCGCGCUCuuGGC--CGGCGCc -3' miRNA: 3'- cAGCa--GGUGUGAGggCUGuuGCUGCG- -5' |
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30940 | 3' | -54.4 | NC_006552.1 | + | 5257 | 0.7 | 0.630695 |
Target: 5'- gGUCGUCgCACGCcugccgguaguUgCCGGCGagcaagucGCGGCGCa -3' miRNA: 3'- -CAGCAG-GUGUG-----------AgGGCUGU--------UGCUGCG- -5' |
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30940 | 3' | -54.4 | NC_006552.1 | + | 25699 | 0.69 | 0.684565 |
Target: 5'- aGUCccaugCCGCACUCCUGcGCAGCGAUu- -3' miRNA: 3'- -CAGca---GGUGUGAGGGC-UGUUGCUGcg -5' |
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30940 | 3' | -54.4 | NC_006552.1 | + | 64707 | 0.65 | 0.847302 |
Target: 5'- -aUGaCCGCGCUCaugcgcaugcaaGACGACGugGCg -3' miRNA: 3'- caGCaGGUGUGAGgg----------CUGUUGCugCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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