Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30952 | 3' | -55.3 | NC_006556.1 | + | 15598 | 0.66 | 0.454457 |
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Target: 5'- aGGCCuagcGCUAUAgGCCACGAUugauacaaagGUC-CCa -3' miRNA: 3'- gCCGG----UGAUAU-CGGUGCUG----------CAGuGGa -5' |
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| 30952 | 3' | -55.3 | NC_006556.1 | + | 4356 | 0.66 | 0.413135 |
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Target: 5'- aGGCCAU---GGCCAUGGgGUCcCCc -3' miRNA: 3'- gCCGGUGauaUCGGUGCUgCAGuGGa -5' |
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| 30952 | 3' | -55.3 | NC_006556.1 | + | 11109 | 0.67 | 0.393362 |
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Target: 5'- uGGCCAC---AGCCGCGcCuaUCACCUu -3' miRNA: 3'- gCCGGUGauaUCGGUGCuGc-AGUGGA- -5' |
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| 30952 | 3' | -55.3 | NC_006556.1 | + | 4943 | 0.67 | 0.364879 |
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Target: 5'- cCGG-CACUAUAuacGCCAUGGCGUaaagGCCg -3' miRNA: 3'- -GCCgGUGAUAU---CGGUGCUGCAg---UGGa -5' |
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| 30952 | 3' | -55.3 | NC_006556.1 | + | 3556 | 0.69 | 0.296115 |
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Target: 5'- -uGCCAUaAUGGUCACGACGgccuaCGCCa -3' miRNA: 3'- gcCGGUGaUAUCGGUGCUGCa----GUGGa -5' |
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| 30952 | 3' | -55.3 | NC_006556.1 | + | 15365 | 0.71 | 0.206537 |
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Target: 5'- aGGCCGCUAUGGCCAguaucgcuauuaUGGCcaaCGCCg -3' miRNA: 3'- gCCGGUGAUAUCGGU------------GCUGca-GUGGa -5' |
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| 30952 | 3' | -55.3 | NC_006556.1 | + | 5159 | 0.73 | 0.154503 |
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Target: 5'- uGGCCAUUcacAGUCACGACaUCACCg -3' miRNA: 3'- gCCGGUGAua-UCGGUGCUGcAGUGGa -5' |
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| 30952 | 3' | -55.3 | NC_006556.1 | + | 10911 | 0.73 | 0.137232 |
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Target: 5'- uGGCgC-CUAUGGUCAUGACGUUGCCc -3' miRNA: 3'- gCCG-GuGAUAUCGGUGCUGCAGUGGa -5' |
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| 30952 | 3' | -55.3 | NC_006556.1 | + | 4530 | 1.08 | 0.000311 |
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Target: 5'- uCGGCCACUAUAGCCACGACGUCACCUg -3' miRNA: 3'- -GCCGGUGAUAUCGGUGCUGCAGUGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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